Mouse Anti-NHP ATP5F1A Recombinant Antibody (VS-1024-XY39)
CAT#: VS-1024-XY39
This mouse-derived monoclonal antibody specifically targets ATP5F1A and exhibits reactivity with human, mouse, non-human primate, and rat samples. The immunogen is a purified recombinant fragment of human ATP5F1A, ensuring high specificity and accuracy. It is validated for use in Western blot (WB), immunohistochemistry (IHC), and ELISA, making it a versatile and reliable tool for diverse applications, from protein detection to cellular localization studies. Its robust performance across various assays positions it as a key reagent for comprehensive ATP5F1A research.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13067/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_A_6_6.jpg
Normal Tissue
Figure 7 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_B_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44202/126704_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152234-ATP5F1A
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Specifications
- Immunogen
- Purified recombinant fragment of human ATP5F1A
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ATP5F1A
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB, IHC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
- CAT
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Secondary Antibody
- CAT
- Product Name
See other products for "ATP5F1A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0560J | Rabbit Anti-ATP5F1A Recombinant Antibody (clone 5G11) | ELISA, WB, IHC | Rabbit IgG |
| VS3-WK551 | Rabbit Anti-ATP5F1A Recombinant Antibody (clone JM110-04) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS7-HM173 | Mouse Anti-ATP5F1A Recombinant Antibody (clone 1A5B6) | WB, IHC, FCM | Mouse IgG1 |
| VS7-HM174 | Mouse Anti-ATP5F1A Recombinant Antibody (clone 3A6B9) | WB, IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY621 | Anti-ATP5F1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY622 | Anti-Mouse ATP5F1A Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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