Rabbit Anti-NHP AXL Recombinant Antibody (VS-1024-XY44)

Name: Rabbit Anti-NHP AXL Recombinant Antibody (VS-1024-XY44)
SKU: VS-1024-XY44
Rating: 5 (1 reviews)

CAT#: VS-1024-XY44

This rabbit-derived monoclonal antibody is meticulously designed to target AXL, demonstrating reactivity with human and non-human primate samples. It utilizes a recombinant human fragment of the Axl protein as the immunogen, ensuring precise and reliable detection. Suitable for Western blot (WB), immunofluorescence (IF), and immunoprecipitation (IP), this antibody offers versatile applications for in-depth studies of AXL. Its exceptional specificity and broad utility make it an essential reagent for comprehensive research into AXL's role in various biological processes.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, actin filaments & vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75217/1899_C1_1_selected.jpg

Normal Tissue Figure 2 IHC staining of human thyroid gland

Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32501/ihc_selected.jpg

Normal Tissue Figure 3 Skeletal muscle

Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_B_1_6.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_8_3.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_9_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_4_6.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (fHDF/TERT166, U-251 MG)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167601-AXL

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Specifications

Immunogen
Recombinant human fragment of the AXL protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
AXL

Species Reactivity
Human, Non-human primate

Applications
WB, IF, IP

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
WB: 1:800, IF: 5 µg, IP: 1:50

Target

Alternative Names
ARK; AXL3; JTK11; Tyro7; UFO

Gene ID
558

UniProt ID
P30530

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY44.pdf

MSDS

MSDS - VS-1024-XY44.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Secondary Antibody

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