Mouse Anti-NHP CCNA2 Recombinant Antibody (clone E23.1) (CAT#: VS-1024-XY145)
This monoclonal antibody, derived from mouse, is designed to detect CCNA2 with remarkable precision, showing reactivity across bovine, human, mouse, non-human primate, and rat. The antibody is based on a bovine Cyclin A fusion protein as the immunogen, ensuring robust specificity. It is validated for use in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP), making it an essential reagent for detailed analysis of CCNA2 in various experimental contexts.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/667_H11_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/667_H11_1_selected.jpg
Figure 2 Tonsil
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_6_8_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_6_8_selected.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_5.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_9_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_9_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145386-CCNA2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145386-CCNA2
Specifications
- Immunogen
- Bovine Cyclin A fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- CCNA2
- Species Reactivity
- Bovine, Human, Mouse, Non-human primate, Rat
- Clone
- E23.1
- Applications
- WB, IHC, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:50-1:500, IF: 1:50, IP: 1 µg
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0513 | Hi-Affi™ Rabbit Anti-CCNA2 Recombinant Antibody (clone DS513AB) | IF, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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