Mouse Anti-NHP CCNA2 Recombinant Antibody (clone E23.1)
CAT#: VS-1024-XY145
This monoclonal antibody, derived from mouse, is designed to detect CCNA2 with remarkable precision, showing reactivity across bovine, human, mouse, non-human primate, and rat. The antibody is based on a bovine Cyclin A fusion protein as the immunogen, ensuring robust specificity. It is validated for use in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP), making it an essential reagent for detailed analysis of CCNA2 in various experimental contexts.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/667_H11_1_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_6_8_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145386-CCNA2
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Specifications
- Immunogen
- Bovine Cyclin A fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- CCNA2
- Species Reactivity
- Bovine, Human, Mouse, Non-human primate, Rat
- Clone
- E23.1
- Applications
- WB, IHC, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:50-1:500, IF: 1:50, IP: 1 µg
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CCNA2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3730z | Mouse Anti-CCNA2 Recombinant Antibody (clone 1F1) | WB, IHC, IF, ELISA | Mouse IgG2a |
| MRO-0408-CN | Mouse Anti-CCNA2 Recombinant Antibody (clone N0-E3) | WB, IF, IHC | Mouse IgG1 |
| MRO-0409-CN | Rabbit Anti-CCNA2 Recombinant Antibody (clone CBACN-161) | WB, IF, IHC | Rabbit IgG |
| VS3-QX172 | Mouse Anti-CCNA2 Recombinant Antibody (clone 1G1-C7-F9) | WB, IHC, ICC, FC, ELISA | |
| VS7-HM312 | Mouse Anti-CCNA2 Recombinant Antibody (clone 6B4D11) | WB, IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0513 | Hi-Affi™ Rabbit Anti-CCNA2 Recombinant Antibody (clone DS513AB) | IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4366 | PE-B*40:01/Human CCNA2 (YHEDIHTYL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1313 | AbPlus™ Anti-Ccna2 Magnetic Beads (VS-0724-YC1313) | IP, Protein Purification | |
| VS-0724-YC1370 | AbPlus™ Anti-CCNA2 Magnetic Beads (VS-0724-YC1370) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY333 | Anti-CCNA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1079 | Anti-Mouse CCNA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1078 | Anti-Human CCNA2 Immunohistochemistry Kit | IHC |
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