Mouse Anti-NHP CCNB1 Recombinant Antibody (clone V152) (CAT#: VS-1024-XY146)
This highly specific mouse monoclonal antibody is designed to recognize CCNB1 with exceptional sensitivity. It shows cross-reactivity with hamster, human, mouse, non-human primate, and rat, reflecting its broad applicability. Developed using hamster Cyclin B1 as the immunogen, it supports diverse research needs, including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Ideal for in-depth studies of cell cycle dynamics and cancer biology, this antibody ensures reliable and accurate results across multiple platforms.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
Specifications
- Immunogen
- Hamster Cyclin B1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CCNB1
- Species Reactivity
- Hamster, Human, Mouse, Non-human primate, Rat
- Clone
- V152
- Applications
- WB, IHC, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 30% glycerol, 1mg/mL BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:10-1:20, IF: 1:50-1:500, IP: 1 µg
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1647z | Mouse Anti-CCNB1 Recombinant Antibody (clone 28C1) | WB, IF, IHC, IP, FuncS | Mouse IgG1, κ |
| VS3-QX173 | Mouse Anti-CCNB1 Recombinant Antibody (clone 1B3-3B1-1B4) | IHC | Mouse IgG |
| VS3-QX174 | Mouse Anti-CCNB1 Recombinant Antibody (clone 4G5-3F3-9G9) | IHC | Mouse IgG |
| VS7-HM313 | Mouse Anti-CCNB1 Recombinant Antibody (clone CBL103HM) | WB, IF, ICC, FCM, ELISA | Mouse IgG |
| VS7-HM314 | Mouse Anti-CCNB1 Recombinant Antibody (clone CBL104HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
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