Mouse Anti-NHP CCND3 Recombinant Antibody (clone DCS-22)
CAT#: VS-1024-XY149
This mouse-origin monoclonal antibody is meticulously designed against CCND3, exhibiting reactivity with human, mouse, non-human primate, and rat samples. The antibody is generated using purified recombinant full-length cyclin D3 protein as the immunogen, ensuring precise specificity. It is optimized for use in Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) applications. Its broad reactivity and robust performance across multiple experimental techniques make it an invaluable asset for in-depth analysis and research into cyclin D3 functions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42871/1432_F9_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_9_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in granular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_8_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (MOLT-4, REH)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112576-CCND3
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Specifications
- Immunogen
- Purified human recombinant full length cyclin D3 protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CCND3
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- DCS-22
- Applications
- WB, IHC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.2% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1 µg/mL, IHC: 1:25, IP: 2 µg/mL
Target
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Related Signaling Pathways
Hedgehog Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0111CT | Mouse Anti-CCND3 Recombinant Antibody (clone 7I5) | ELISA, WB | Mouse IgG1 |
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| ZG-0793F | Mouse Anti-CCND3 Recombinant Antibody (clone DCS2.2) | WB, IP, IHC | Mouse IgG1 |
| VS3-CJ865 | Rabbit Anti-CCND3 Recombinant Antibody (VS3-CJ865) | WB, ICC, IF, FC | Rabbit IgG |
| VS7-HM316 | Mouse Anti-CCND3 Recombinant Antibody (clone CBL106HM) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-265CQ | Mouse Anti-CCND3 Recombinant Antibody (clone CBL926) | FC, IF, IHC, IP, WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0516 | Hi-Affi™ Rabbit Anti-CCND3 Recombinant Antibody (clone DS516AB) | FC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY46 | AbPlus™ Anti-CCND3 Magnetic Beads (DCS2.2) | IP, Protein Purification | |
| VS-0724-YC1228 | AbPlus™ Anti-CCND3 Magnetic Beads (VS-0724-YC1228) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY336 | Anti-CCND3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1089 | Anti-Mouse CCND3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1088 | Anti-Human CCND3 Immunohistochemistry Kit | IHC |
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