Mouse Anti-NHP CCND3 Recombinant Antibody (clone DCS-22) (CAT#: VS-1024-XY149)
This mouse-origin monoclonal antibody is meticulously designed against CCND3, exhibiting reactivity with human, mouse, non-human primate, and rat samples. The antibody is generated using purified recombinant full-length cyclin D3 protein as the immunogen, ensuring precise specificity. It is optimized for use in Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) applications. Its broad reactivity and robust performance across multiple experimental techniques make it an invaluable asset for in-depth analysis and research into cyclin D3 functions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42871/1432_F9_3_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42871/1432_F9_3_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_9_5.jpg
Figure 3 Cerebellum
(Cells in granular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_8_8.jpg
(Cells in granular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_B_8_8.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_9_3.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_5_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/116/2169_A_7_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (MOLT-4, REH)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112576-CCND3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112576-CCND3
Specifications
- Immunogen
- Purified human recombinant full length cyclin D3 protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CCND3
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- DCS-22
- Applications
- WB, IHC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.2% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1 µg/mL, IHC: 1:25, IP: 2 µg/mL
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Hedgehog Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-265CQ | Mouse Anti-CCND3 Recombinant Antibody (clone CBL926) | FC, IF, IHC, IP, WB, Neut | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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