Rat Anti-NHP CD8A Recombinant Antibody (clone YTC182.20)
CAT#: VS-1024-XY112
The rat-derived monoclonal antibody is engineered to precisely target CD8A and is proven to react with human and non-human primate samples. Designed with flow cytometry (FC) in mind, it provides reliable and reproducible results in cell surface marker detection. The antibody's specificity and consistent performance in identifying CD8A-expressing cells make it an invaluable resource for immunological studies, particularly those focused on T-cell populations across different species. Its optimized design enhances both sensitivity and precision in flow cytometry applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
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Specifications
- Immunogen
- Murine L cells transfected with human CD8 gene
- Host Species
- Rat
- Type
- Rat IgG2b
- Specificity
- CD8A
- Species Reactivity
- Human, Non-human primate
- Clone
- YTC182.20
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 1:100-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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