Mouse Anti-NHP CEACAM5 Recombinant Antibody (clone SPM506) (CAT#: VS-1024-XY62)
Harnessing the precision of a mouse-derived monoclonal antibody, this reagent targets CEACAM5 with remarkable specificity for human and non-human primate tissues. The immunogen utilized is a human colon carcinoma extract, ensuring accurate and relevant detection. Suitable for immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC), this antibody offers comprehensive capabilities for studying CEACAM5 expression. Its robustness and versatility make it an invaluable tool for research in oncology and cell biology.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
Figure 4 Rectum
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
Specifications
- Immunogen
- Human colon carcinoma extract
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CEACAM5
- Species Reactivity
- Human, Non-human primate
- Clone
- SPM506
- Applications
- IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 1-2 µg/mL, IF: 1-2 µg/mL, FC: 0.5-1 µg/106 cells
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-080 | Recombinant Anti-human CEACAM5 VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-002LC-VHH | Anti-Human CEACAM5 Recombinant Antibody(VHH library) | SPR, FC, ELISA | Single domain antibody |
| HPAB-0204-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0204-FY) | ADCC, ELISA | llama VHH |
| HPAB-0205-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0205-FY) | ADCC, E | llama VHH |
| HPAB-1024WJ | Recombinant Camelid Anti-CEACAM5 Single Domain Antibody | SPR, Inhib | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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