Mouse Anti-NHP CEACAM5 Recombinant Antibody (clone SPM506)

Name: Mouse Anti-NHP CEACAM5 Recombinant Antibody (clone SPM506)
SKU: VS-1024-XY62
Rating: 5 (1 reviews)

CAT#: VS-1024-XY62

Harnessing the precision of a mouse-derived monoclonal antibody, this reagent targets CEACAM5 with remarkable specificity for human and non-human primate tissues. The immunogen utilized is a human colon carcinoma extract, ensuring accurate and relevant detection. Suitable for immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC), this antibody offers comprehensive capabilities for studying CEACAM5 expression. Its robustness and versatility make it an invaluable tool for research in oncology and cell biology.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HaCaT

Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg

Subcellular Location Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg

Normal Tissue Figure 3 Colon

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg

Normal Tissue Figure 4 Rectum

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enriched (OE19)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5

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Specifications

Immunogen
Human colon carcinoma extract

Host Species
Mouse

Type
Mouse IgG1, kappa

Specificity
CEACAM5

Species Reactivity
Human, Non-human primate

Clone
SPM506

Applications
IHC, IF, FC

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.4, with 0.05% BSA

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
IHC: 1-2 µg/mL, IF: 1-2 µg/mL, FC: 0.5-1 µg/10⁶ cells

Target

Alternative Names
CD66e; CEA

Gene ID
1048

UniProt ID
P06731

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY62.pdf

MSDS

MSDS - VS-1024-XY62.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Isotype Control

CAT
Product Name

Secondary Antibody

CAT
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