Mouse Anti-NHP CHD1 Recombinant Antibody (clone 7A2)
CAT#: VS-1024-XY175
The mouse-origin monoclonal antibody is finely tuned to recognize CHD1, exhibiting reactivity with human, mouse and non-human primate samples. The immunogen for this antibody is purified recombinant fragment of human CDH1, ensuring precise targeting. Tailored for WB and ELISA applications, this antibody offers reliable performance in the analysis of CHD1 expression.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/1401_A9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/1158_A9_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/1252_E8_2_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/109218_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/109218_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/109218_A_8_5.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22236/109218_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153922-CHD1
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Specifications
- Immunogen
- Purified recombinant fragment of human CDH1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CHD1
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 7A2
- Applications
- WB, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, ELISA: 1:8000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
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See other products for "CHD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-295 | Human Anti-CHD1 Recombinant Antibody (clone D627) | IP, IF, ELISA | Human IgG |
| MRO-0356-CN | Mouse Anti-CHD1 Recombinant Antibody (clone 2-2) | WB, IHC | Mouse IgG1 |
| VS3-CJ35 | Mouse Anti-CHD1 Recombinant Antibody (VS3-CJ35) | WB, ELISA | Mouse IgG |
| VS-0625-FY1451 | Human Anti-CHD1 Recombinant Antibody (clone 2506W1346) | ELISA, IP MS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY442 | Anti-CHD1 Immunohistochemistry Kit | IHC |
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