Mouse Anti-NHP CHEK2 Recombinant Antibody (clone 1C12B8)
CAT#: VS-1024-XY121
Featuring a mouse-derived monoclonal format, this antibody is tailored to target CHEK2 with exceptional specificity. It exhibits reactivity with both human and non-human primate samples, making it suitable for Western blot (WB) applications. The antibody ensures accurate detection and analysis of CHEK2, providing critical insights into cellular stress mechanisms and related pathological conditions. Its high performance renders it an indispensable tool for advanced research and diagnostic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
Normal Tissue
Figure 2 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
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Specifications
- Immunogen
- Purified recombinant fragment of human CHEK2
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- CHEK2
- Species Reactivity
- Human, Non-human primate
- Clone
- 1C12B8
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.5-2 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
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Secondary Antibody
- CAT
- Product Name
See other products for "CHEK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0122MZ | Chicken Anti-CHEK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0689 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone DS689AB) | DB, IP, WB | Rabbit IgG |
| MOR-4518 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone TH26DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0360-CN | Rabbit Anti-CHEK2 Recombinant Antibody (clone CBACN-147) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0420F | Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-QX235 | Mouse Anti-CHEK2 Recombinant Antibody (clone 4B1-C5-B3) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-CJ199 | Rabbit Anti-CHEK2 Recombinant Antibody (VS3-CJ199) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-WK2072 | Rabbit Anti-CHEK2 (pT68) Recombinant Antibody (clone R23-2L-9) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC455 | AbPlus™ Anti-CHEK2 Magnetic Beads (VS-0724-YC455) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY446 | Anti-CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1430 | Anti-Mouse CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1431 | Anti-Canine CHEK2 Immunohistochemistry Kit | IHC |
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