Mouse Anti-NHP CRTC2 Recombinant Antibody (clone 5B10)
CAT#: VS-1024-XY509
Targeting CRTC2 with precision, this monoclonal antibody demonstrates excellent reactivity towards human and non-human primate specimens. The immunogen, a purified recombinant fragment of human CRTC2, ensures high specificity and effectiveness across a range of applications, including Western blotting, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA. Its versatile functionality makes it an indispensable tool for researchers investigating the regulatory mechanisms of CRTC2 in cellular signaling and metabolic pathways. With its robust performance, this antibody is ideal for advancing understanding in various fields of biological research.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28454/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/507_C8_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160741-CRTC2
❮
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Specifications
- Immunogen
- Purified recombinant fragment of human CRTC2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CRTC2
- Species Reactivity
- Human, Non-human primate
- Clone
- 5B10
- Applications
- WB, IHC, IF, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 5B10"
- CAT
- Product Name
See other products for "CRTC2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1814MZ | Recombinant Mouse Anti-Human CRTC2 Antibody | ELISA, WB | Mouse antibody |
| VS3-XY530 | Mouse Anti-CRTC2 Recombinant Antibody (clone 5B10) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS7-HM775 | Mouse Anti-CRTC2 Recombinant Antibody (clone CBL178HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0814 | Hi-Affi™ Rabbit Anti-CRTC2 Recombinant Antibody (clone DS814AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY550 | Anti-CRTC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1681 | Anti-Human CRTC2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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