Mouse Anti-NHP CRTC2 Recombinant Antibody (clone 5B10) (CAT#: VS-1024-XY509)
Targeting CRTC2 with precision, this monoclonal antibody demonstrates excellent reactivity towards human and non-human primate specimens. The immunogen, a purified recombinant fragment of human CRTC2, ensures high specificity and effectiveness across a range of applications, including Western blotting, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA. Its versatile functionality makes it an indispensable tool for researchers investigating the regulatory mechanisms of CRTC2 in cellular signaling and metabolic pathways. With its robust performance, this antibody is ideal for advancing understanding in various fields of biological research.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28454/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28454/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/507_C8_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/507_C8_1_selected.jpg
Figure 3 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/ihc_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_9_8.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_B_7_5.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_9_3.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_8_5.jpg
Figure 8 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_5_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28465/59214_A_5_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160741-CRTC2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160741-CRTC2
Specifications
- Immunogen
- Purified recombinant fragment of human CRTC2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CRTC2
- Species Reactivity
- Human, Non-human primate
- Clone
- 5B10
- Applications
- WB, IHC, IF, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1814MZ | Recombinant Mouse Anti-Human CRTC2 Antibody | ELISA, WB | Mouse antibody |
| VS3-XY530 | Mouse Anti-CRTC2 Recombinant Antibody (clone 5B10) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS7-HM775 | Mouse Anti-CRTC2 Recombinant Antibody (clone CBL178HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
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