Mouse Anti-NHP CTNNB1 Recombinant Antibody (clone 15B8) (CAT#: VS-1024-XY50)
Introducing a mouse-derived monoclonal antibody targeting CTNNB1 (beta-catenin), designed to deliver superior reactivity across human, mouse, non-human primate, and rat samples. The antibody is generated using a recombinant beta-catenin protein as the immunogen, ensuring exceptional specificity and performance. Suitable for a range of applications including Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody provides reliable and consistent results for studying beta-catenin dynamics in various experimental contexts. Its broad reactivity and application versatility make it a pivotal tool for advanced research.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/if_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/ihc_selected.jpg
Figure 3 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/108/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/108/ihc_selected.jpg
Figure 4 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1950/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1950/ihc_selected.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_B_8_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_4.jpg
Figure 8 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_1_3.jpg
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_1_3.jpg
Figure 9 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_5.jpg
(Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_5.jpg
Figure 10 Testis
(Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_4_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_4_6.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168036-CTNNB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168036-CTNNB1
Specifications
- Immunogen
- Recombinant beta Catenin protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- CTNNB1
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 15B8
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:800, IHC: 1:100-1:800, IF: 1:50-1:800
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-247-F(E) | Recombinant Anti-human CTNNB1 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-247-F(E) | Recombinant Human Anti-human CTNNB1 Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| PSBZ-140 | Mouse Anti-CTNNB1 Recombinant Antibody (clone BC-22); scFv Fragment | ELISA, Block | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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