Mouse Anti-NHP DEF6 Recombinant Antibody (clone OTI1E3) (CAT#: VS-1024-XY160)
This monoclonal antibody from mouse is designed to recognize DEF6 with reactivity across dog, human, mouse, non-human primate, and rat species. It employs a full-length recombinant human DEF6 protein as its immunogen to ensure high specificity. Ideal for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications, this antibody is a robust tool for detailed analysis of DEF6 expression and distribution in various research and diagnostic settings.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38975/1396_C7_3_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38975/1396_C7_3_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38976/81482_A_7_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38976/81482_A_7_3.jpg
Figure 3 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38976/81482_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38976/81482_A_9_8.jpg
Figure 4 Spleen
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38976/81482_B_7_4.jpg
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38976/81482_B_7_4.jpg
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, NB-4, REH, THP-1, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000023892-DEF6
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000023892-DEF6
Specifications
- Immunogen
- Full length human recombinant protein of DEF6
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- DEF6
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI1E3
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, IF: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1084MZ | Recombinant Mouse Anti-Human DEF6 Antibody | IHC-P | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
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