Mouse Anti-NHP DHFR Recombinant Antibody (clone OTI6G7) (CAT#: VS-1024-XY163)
Derived from mouse, this monoclonal antibody specifically recognizes DHFR and exhibits reactivity with dog, human, and non-human primate tissues. It is optimized for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications, providing reliable performance across diverse species. Its comprehensive reactivity and utility make it a critical reagent for detailed investigations into DHFR expression and distribution.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
Specifications
- Immunogen
- Full-length protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- DHFR
- Species Reactivity
- Dog, Human, Non-human primate
- Clone
- OTI6G7
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:20, IHC: 1:50, IF: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DHFR"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Single Domain Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812z | Mouse Anti-DHFR Recombinant Antibody (clone 30C7) | WB, ICC, IHC | Mouse IgG2b |
| MOB-0052CT | Recombinant Mouse anti-Human DHFR Monoclonal antibody (4B22) | IHC-P, WB | |
| ZG-0636J | Rabbit Anti-DHFR Recombinant Antibody (clone 9B2) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-XY3331 | Rabbit Anti-DHFR Recombinant Antibody (clone R05-2C7) | WB, IHC-P, ICC, IF | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-1024-XY163. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Neut, FuncS
Application: Neut, ELISA, FuncS
-YJ1.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, Inhib, FuncS
Application: ELISA, FC, IF, IHC, WB
Application: ELISA, WB
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
