Mouse Anti-NHP GAPDH Recombinant Antibody (clone 1D4)

CAT#: VS-1024-XY212

Designed to target GAPDH with exceptional specificity, this mouse monoclonal antibody demonstrates broad reactivity across species, including fruit fly, horse, cat, human, mouse, non-human primate, pig, rabbit, rat, and zebrafish. With proven performance in Western blot (WB) and immunofluorescence (IF), it is an essential tool for studying GAPDH expression in a wide range of biological samples, making it ideal for both fundamental research and more complex diagnostic investigations.

Gene Expression
Figure 1 IF staining of human cell line U-251 MG Figure 2 IF staining of human cell line U-2 OS Figure 3 IHC staining of human stomach Figure 4 IHC staining of human skin Figure 5 IHC staining of human kidney Figure 6 IHC staining of human skeletal muscle Figure 7 IF staining of human cell line A549 Figure 8 Colon Figure 9 Liver Figure 10 Kidney Figure 11 Testis Figure 12 Lymph node Figure 13 RNA cell line category: Low cell line specificity

Specifications

  • Immunogen
  • Extensively purified pig GAPDH
  • Host Species
  • Mouse
  • Type
  • Mouse IgM
  • Specificity
  • GAPDH
  • Species Reactivity
  • Fruit fly, Horse, Cat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Zebrafish
  • Clone
  • 1D4
  • Applications
  • WB, IF

Product Property

  • Purification
  • Protein A purified
  • Purity
  • >95%, determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • PBS with 50% glycerol
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • WB: 1:800, IF: 1:100

Target

  • Alternative Names
  • Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12;6 GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase; 38 Kd Component; Aging-Associated Gene 9 Protein;
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

Isotype Control

Secondary Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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