Mouse Anti-NHP GRB10 Recombinant Antibody (clone 5H7) (CAT#: VS-1024-XY232)
Optimized for the detection of GRB10, this mouse monoclonal antibody shows reactivity with human, non-human primate, and rat tissues. The immunogen is an E. coli-derived recombinant human GRB10 protein, ensuring high specificity and reliability. Suitable for Western blot (WB) and flow cytometry (FC), this antibody offers dependable performance for studying GRB10 expression and function across various experimental models.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nuclear membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31818/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nuclear membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31818/if_selected.jpg
Figure 2 Skeletal muscle
(High expression in skeletal muscle)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27502/63878_B_1_6_val_selected.jpg
(High expression in skeletal muscle)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27502/63878_B_1_6_val_selected.jpg
Figure 3 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/ihc_selected.jpg
Figure 4 Endometrium
(Cells in endometrial stroma Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_B_6_3.jpg
(Cells in endometrial stroma Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_B_6_3.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_A_9_8.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_A_9_4.jpg
Figure 7 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19423/43440_A_8_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106070-GRB10
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106070-GRB10
Specifications
- Immunogen
- E. coli-derived human GRB10 recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- GRB10
- Species Reactivity
- Human, Non-human primate, Rat
- Clone
- 5H7
- Applications
- WB, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Lyophilized
- Buffer
- PBS with 4 mg trehalose
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.25-0.5 µg/mL, FC: 1-3 µg/106 cells
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
mTOR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2572z | Mouse Anti-GRB10 Recombinant Antibody (clone 26B3) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| MOB-1409MZ | Recombinant Mouse Anti-Human GRB10 Antibody (clone 1A7) | WB | Mouse antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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