Rat Anti-NHP HSF1 Recombinant Antibody (clone 10H8)
CAT#: VS-1024-XY260
Developed for the detection of HSF1, this rat-derived monoclonal antibody demonstrates broad reactivity with bovine, guinea pig, hamster, human, mouse, non-human primate, rabbit, and rat specimens. The immunogen is a purified recombinant mouse HSF1 protein, ensuring precise targeting. Suitable for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody provides reliable performance for studying HSF1 expression and localization across a wide range of species and applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8888/41_C11_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_7_5.jpg
Normal Tissue
Figure 5 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_4_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185122-HSF1
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Specifications
- Immunogen
- Purified recombinant mouse HSF1 protein
- Host Species
- Rat
- Type
- Rat IgG1
- Specificity
- HSF1
- Species Reactivity
- Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat
- Clone
- 10H8
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 47.45 mM phosphate/2.15 mM MES, pH 7.4, with 2 mM EDTA, 0.17066 µM Pentachlorophenol, 0.8 mM ethanol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:800, IF: 1:200
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1134MZ | Recombinant Mouse Anti-Human HSF1 Antibody (clone K8G0) | ELISA | Mouse antibody |
| MRO-2336-CN | Rabbit Anti-HSF1 Recombinant Antibody (clone CBACN-627) | WB, IF, IHC, IP, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1685 | Rabbit Anti-HSF1 Recombinant Antibody (clone DS1685AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1065 | Anti-HSF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3294 | Anti-Mouse HSF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3293 | Anti-Human HSF1 Immunohistochemistry Kit | IHC |
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