Rat Anti-NHP HSF2 Recombinant Antibody (clone 3E2) (CAT#: VS-1024-XY261)
Developed to target HSF2, this rat-derived monoclonal antibody exhibits reactivity with human, mouse, and non-human primate samples. The immunogen utilized is a recombinant mouse HSF2 protein, ensuring precise antigen recognition. Suitable for Western blot (WB) and immunoprecipitation (IP) applications, this antibody offers reliable performance for studying HSF2 expression and interactions across a range of biological contexts.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31455/if_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31455/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate nuclear positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31455/ihc_selected.jpg
(Immunohistochemical staining of human testis shows moderate nuclear positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31455/ihc_selected.jpg
Figure 3 IHC staining of human testis
(Immunohistochemical staining of human testis shows distinct nuclear positivity in spermatogonia.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/ihc_selected.jpg
(Immunohistochemical staining of human testis shows distinct nuclear positivity in spermatogonia.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/ihc_selected.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/40996_A_6_6.jpg
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/40996_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear Non-germinal center cells)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/40996_A_9_8.jpg
(Germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear Non-germinal center cells)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/40996_A_9_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000025156-HSF2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000025156-HSF2
Specifications
- Immunogen
- Recombinant mouse HSF2 protein
- Host Species
- Rat
- Type
- Rat IgG1
- Specificity
- HSF2
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 3E2
- Applications
- WB, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 10 mM PBS, pH 7.4, with 0.2% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:200, IP: 1:400
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1135MZ | Recombinant Mouse Anti-Human HSF2 Antibody (clone 832E2) | WB | Mouse antibody |
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