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    Rat Anti-NHP HSP90B1 Recombinant Antibody (clone SPM249) (CAT#: VS-1024-XY235)

    Designed to target HSP90B1, this rat-derived monoclonal antibody demonstrates broad reactivity across bovine, dog, horse, hamster, human, mouse, non-human primate, sheep, rabbit, and rat species. The antibody is validated for use in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC). Its extensive cross-species reactivity and versatile applications make it a valuable tool for comprehensive studies of HSP90B1 expression and function in various experimental and diagnostic contexts.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/if_selected.jpg
    Figure 2 IF staining of human cell line A431
    Figure 2 IF staining of human cell line A431
    (Immunofluorescent staining of human cell line A431 shows localization to endoplasmic reticulum.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/14_F12_1_blue_red_green.jpg
    Figure 3 IF staining of human cell line U-251 MG
    Figure 3 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/16_F12_1_blue_red_green.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_7_5.jpg
    Figure 5 Thyroid gland
    Figure 5 Thyroid gland
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_3_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Bowman's capsule
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Collecting ducts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Distal tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Proximal tubules (cell body)
    Staining:High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_7_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Pachytene spermatocytes
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_6_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166598-HSP90B1

    Specifications

    • Host Species
    • Rat
    • Type
    • Rat IgG2a, kappa
    • Specificity
    • HSP90B1
    • Species Reactivity
    • Bovine, Dog, Horse, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat
    • Clone
    • SPM249
    • Applications
    • WB, IHC, IF, FC

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4, with 0.05% BSA
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • WB: 1-2 µg/mL, IHC: 1-2 µg/mL, IF: 1-2 µg/mL, FC: 1-2 µg/106 cells

    Target

    • Alternative Names
    • ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "HSP90B1"

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    CAT Product Name Application Type
    NAB-1330-VHH Recombinant Anti-Human HSP90B1 VHH Single Domain Antibody FUNC, FA, IP, ChiP Llama VHH

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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