Mouse Anti-NHP HSPA4 Recombinant Antibody (clone 3A11)
CAT#: VS-1024-XY264
Specially developed for the detection of HSPA4, this mouse monoclonal antibody exhibits reactivity with human and non-human primate samples. Its robustness in Western blot (WB) assays ensures accurate and reliable analysis of HSPA4 expression. Designed for precise performance, this antibody is an essential tool for research into the functional roles and regulatory mechanisms of HSPA4 across various biological systems.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/100_C10_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_6_6_selected.jpg
Subcellular Location
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25529/60475_A_1_5_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/101_C10_1_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/82_C10_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/25173_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_7_3.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170606-HSPA4
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, kappa
- Specificity
- HSPA4
- Species Reactivity
- Human, Non-human primate
- Clone
- 3A11
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 3-5 µg/mL
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 4; Heat Shock 70-Related Protein APG-2; Heat Shock 70kDa Protein 4; Heat Shock 70kD Protein 4; Hsp70 RY; Hsp70RY; Epididymis Secretory Sperm Binding Protein Li 5a; Heat Shock 70 KDa Protein 4; Heat Shock Protein; 110 KDa;
- Gene ID
- 3308
- UniProt ID
- P34932
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1694 | Rabbit Anti-HSPA4 Recombinant Antibody (clone DS1694AB) | WB, IP, ICC, ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-0787-CN | Rabbit Anti-HSPA4 Recombinant Antibody (clone CBACN-296) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-A1209-YJ | Human Anti-HSPA4 Recombinant Antibody (clone G6A) | ELISA, IF | Human IgG |
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| VS3-XY830 | Mouse Anti-HSPA4 Recombinant Antibody (clone 5F2D10) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS4-WK231 | Mouse Anti-HSPA4 Recombinant Antibody (VS4-WK231) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC698 | AbPlus™ Anti-HSPA4 Magnetic Beads (VS-0724-YC698) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3324 | Anti-HSPA4 Immunohistochemistry Kit | IHC |
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