Mouse Anti-NHP IQGAP1 Recombinant Antibody (clone AF1)
CAT#: VS-1024-XY283
Designed to target IQGAP1 with high specificity, this mouse monoclonal antibody demonstrates reactivity with human, mouse, and non-human primate tissues. Developed using a recombinant protein as the immunogen, it ensures precise antigen recognition. Suitable for use in both Western blot (WB) and immunofluorescence (IF), this antibody is a valuable resource for researchers investigating IQGAP1 expression and localization in various biological models.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13302/671_E1_1_selected.jpg
Normal Tissue
Figure 2 Endometrium 1
High expression in endometrium 1
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14055/33083_B_6_3_val_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13302/32877_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13302/32877_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13302/32877_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13302/32877_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13302/32877_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140575-IQGAP1
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Specifications
- Immunogen
- A recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG2b, kappa
- Specificity
- IQGAP1
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- AF1
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-3 µg/mL, IF: 2 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IQGAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0988CT | Recombinant Mouse anti-Human IQGAP1 Monoclonal antibody (EML945) | ICC, IF, IHC-P, WB | |
| MRO-0864-CN | Recombinant Rabbit Anti-IQGAP1 Monoclonal Antibody (JG38-74) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK489 | Rabbit Anti-IQGAP1 Recombinant Antibody (clone JG38-74) | WB, ELISA | Rabbit IgG |
| VS3-XY3395 | Rabbit Anti-IQGAP1 Recombinant Antibody (clone R01-9S9) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1849 | Hi-Affi™ Recombinant Rabbit Anti-IQGAP1 Monoclonal Antibody (DS1849AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1135 | Anti-IQGAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3578 | Anti-Mouse IQGAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3577 | Anti-Human IQGAP1 Immunohistochemistry Kit | IHC |
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