Mouse Anti-NHP IRAK4 Recombinant Antibody (clone 2H9)
CAT#: VS-1024-XY284
Optimized for accurate detection of IRAK4, this mouse monoclonal antibody displays reactivity with human, mouse, and non-human primate samples. Its immunogen, a purified recombinant fragment of human IRAK4, ensures precise binding. Suitable for multiple applications, including Western blot (WB), immunohistochemistry (IHC), flow cytometry (FC), and ELISA, this antibody is a powerful tool for investigating IRAK4 expression and function across various experimental contexts.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/621_E10_5_selected.jpg
Normal Tissue
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_B_8_5.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_1_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/37917_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198001-IRAK4
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Specifications
- Immunogen
- Purified recombinant fragment of human IRAK4
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- IRAK4
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 2H9
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
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Related Signaling Pathways
TLR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRAK4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2079z | Mouse Anti-IRAK4 Recombinant Antibody (clone 27A3) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0992CT | Recombinant Mouse anti-Human IRAK4 Monoclonal antibody (A8A8) | ELISA, IHC-P, WB | |
| ZG-0770J | Rabbit Anti-IRAK4 Recombinant Antibody (clone 10H4) | ELISA, WB, FC | Rabbit IgG |
| VS3-FY761 | Recombinant Rabbit Anti-IRAK4 Antibody (clone R01-1B2) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR149 | Mouse Anti-IRAK4 Monoclonal Antibody (VS7-0425-WR149) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0587CL-S(P) | Mouse Anti-IRAK4 Recombinant Antibody; scFv Fragment (TAB-0587CL-S(P)) | ELISA | Mouse scFv |
| TAB-0587CL-F(E) | Mouse Anti-IRAK4 Recombinant Antibody; Fab Fragment (TAB-0587CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1852 | Hi-Affi™ Recombinant Rabbit Anti-IRAK4 Monoclonal Antibody (DS1852AB) | ICC, WB | IgG |
| MOR-4632 | Hi-Affi™ Recombinant Rabbit Anti-IRAK4 Monoclonal Antibody (TH145DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3582 | Anti-IRAK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3583 | Anti-Human IRAK4 Immunohistochemistry Kit | IHC |
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