Mouse Anti-NHP IRAK4 Recombinant Antibody (clone 2H9) (CAT#: VS-1024-XY284)
Optimized for accurate detection of IRAK4, this mouse monoclonal antibody displays reactivity with human, mouse, and non-human primate samples. Its immunogen, a purified recombinant fragment of human IRAK4, ensures precise binding. Suitable for multiple applications, including Western blot (WB), immunohistochemistry (IHC), flow cytometry (FC), and ELISA, this antibody is a powerful tool for investigating IRAK4 expression and function across various experimental contexts.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, cytosol & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/621_E10_5_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, cytosol & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/621_E10_5_selected.jpg
Figure 3 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/ihc_selected.jpg
Figure 4 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/ihc_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/ihc_selected.jpg
Figure 5 Cerebral cortex
(nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_B_8_5.jpg
(nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_B_8_5.jpg
Figure 6 Pancreas
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_1_3.jpg
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_1_3.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/37917_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/37917_A_7_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_6_6.jpg
Figure 9 RNA cell line category: Cell line enhanced (U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198001-IRAK4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198001-IRAK4
Specifications
- Immunogen
- Purified recombinant fragment of human IRAK4
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- IRAK4
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 2H9
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
TLR Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2079z | Mouse Anti-IRAK4 Recombinant Antibody (clone 27A3) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0992CT | Recombinant Mouse anti-Human IRAK4 Monoclonal antibody (A8A8) | ELISA, IHC-P, WB | |
| ZG-0770J | Rabbit Anti-IRAK4 Recombinant Antibody (clone 10H4) | ELISA, WB, FC | Rabbit IgG |
| VS3-FY761 | Recombinant Rabbit Anti-IRAK4 Antibody (clone R01-1B2) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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