Mouse Anti-NHP LTA4H Recombinant Antibody (clone OTI1E9)
CAT#: VS-1024-XY310
Focused on the detection of LTA4H, this mouse monoclonal antibody demonstrates reactivity with dog, human, non-human primate, and rat tissues. The immunogen is a full-length protein, ensuring precise antigen recognition. Suitable for a variety of applications, including Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP), this antibody serves as a vital tool for investigating LTA4H expression and functionality across different biological systems.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/75_D10_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17017/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15221/37901_A_9_3_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17017/38426_B_7_5.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_A_6_6.jpg
Normal Tissue
Figure 9 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_B_6_4.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15221/37901_A_9_8.jpg
❮
❯
Specifications
- Immunogen
- Full-length protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- LTA4H
- Species Reactivity
- Dog, Human, Non-human primate, Rat
- Clone
- OTI1E9
- Applications
- WB, IHC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:20, IHC: 1:50, IP: 2-4 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| VS-0525-XY4118 | Anti-Mouse LTA4H Immunohistochemistry Kit | IHC | |
| VS-0525-XY4119 | Anti-Rat LTA4H Immunohistochemistry Kit | IHC |
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