Mouse Anti-NHP MCM2 Recombinant Antibody (clone 2B3)
CAT#: VS-1024-XY318
Targeting MCM2, this antibody is designed with a highly specific recognition for epitopes conserved across multiple species, including human, mouse, non-human primates, and rat. Immunized with a purified recombinant fragment of human MCM2, it demonstrates exceptional utility across a range of applications such as Western Blotting, immunohistochemistry, flow cytometry, and ELISA. This broad reactivity makes it a versatile tool for both basic research and translational studies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
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Specifications
- Immunogen
- Purified recombinant fragment of human MCM2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MCM2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 2B3
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 2; Minichromosome Maintenance Protein 2 Homolog; Nuclear Protein BM28; Mitotin; CCNL1; CDCL1; BM28; MCM2 Minichromosome Maintenance Deficient 2; Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin);
- Gene ID
- 4171
- UniProt ID
- P49736
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MCM2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-537-MZ | Mouse Anti-MCM2 Recombinant Antibody (clone CRCT2.1) | WB, IHC-P | |
| VS3-CJ992 | Mouse Anti-MCM2 Recombinant Antibody (clone ABT-MCM2) | WB, IHC-P | Mouse IgG1, κ |
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| VS3-WK2252 | Rabbit Anti-MCM2 (pS41) Recombinant Antibody (VS3-WK2252) | WB, IHC, IP | Rabbit IgG |
| VS3-WK2272 | Rabbit Anti-MCM2 (pS40) Recombinant Antibody (VS3-WK2272) | WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0715MZ | Chicken Anti-MCM2 Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2190 | Hi-Affi™ Recombinant Rabbit Anti-MCM2 Monoclonal Antibody (DS2190AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1319 | Anti-MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4304 | Anti-Mouse MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4303 | Anti-Human MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4305 | Anti-Rat MCM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC711 | CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody (VS13-YC711) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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