Mouse Anti-NHP MCM2 Recombinant Antibody (clone 2B3) (CAT#: VS-1024-XY318)
Targeting MCM2, this antibody is designed with a highly specific recognition for epitopes conserved across multiple species, including human, mouse, non-human primates, and rat. Immunized with a purified recombinant fragment of human MCM2, it demonstrates exceptional utility across a range of applications such as Western Blotting, immunohistochemistry, flow cytometry, and ELISA. This broad reactivity makes it a versatile tool for both basic research and translational studies.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Figure 5 Bone marrow
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
Specifications
- Immunogen
- Purified recombinant fragment of human MCM2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MCM2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 2B3
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 2; Minichromosome Maintenance Protein 2 Homolog; Nuclear Protein BM28; Mitotin; CCNL1; CDCL1; BM28; MCM2 Minichromosome Maintenance Deficient 2; Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin);
- Gene ID
- 4171
- UniProt ID
- P49736
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-537-MZ | Mouse Anti-MCM2 Recombinant Antibody (clone CRCT2.1) | WB, IHC-P | |
| VS3-QX762 | Mouse Anti-MCM2 Recombinant Antibody (clone 2B5-H4-A9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY907 | Recombinant Rabbit Anti-MCM2 (phospho Ser108) Antibody (clone R01-7G9) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY908 | Recombinant Rabbit Anti-MCM2 (phospho Ser40) Antibody (clone R03-1B3) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY909 | Recombinant Rabbit Anti-MCM2 (phospho Ser41) Antibody (clone R07-4C3) | WB, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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