Mouse Anti-NHP NCAM1 Recombinant Antibody (clone MEM-188)
CAT#: VS-1024-XY104
The monoclonal antibody, sourced from mouse, is engineered to recognize NCAM1, displaying notable reactivity with human and non-human primate tissues. Suited for immunohistochemistry (IHC) and flow cytometry (FC) applications, it offers exceptional precision in detecting NCAM1. Its high specificity makes it a critical tool for exploring cellular adhesion and neurodevelopmental studies, ensuring reliability across diverse research settings.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_A_8_3.jpg
Normal Tissue
Figure 4 Soft tissue
Peripheral nerve
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_B_1_7.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL, RH-30, SCLC-21H, SH-SY5Y, U-138 MG, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149294-NCAM1
❮
❯
Specifications
- Immunogen
- KG-1 human acute myelogenous cell line
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- NCAM1
- Species Reactivity
- Human, Non-human primate
- Clone
- MEM-188
- Applications
- IHC, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 1:50, FC: 3 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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