Mouse Anti-NHP NCAM1 Recombinant Antibody (clone MEM-188)

Name: Mouse Anti-NHP NCAM1 Recombinant Antibody (clone MEM-188)
SKU: VS-1024-XY104
Rating: 5 (1 reviews)

CAT#: VS-1024-XY104

The monoclonal antibody, sourced from mouse, is engineered to recognize NCAM1, displaying notable reactivity with human and non-human primate tissues. Suited for immunohistochemistry (IHC) and flow cytometry (FC) applications, it offers exceptional precision in detecting NCAM1. Its high specificity makes it a critical tool for exploring cellular adhesion and neurodevelopmental studies, ensuring reliability across diverse research settings.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/if_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuropil
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_B_9_5.jpg

Normal Tissue Figure 3 Colon

Peripheral nerve/ganglion
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_A_8_3.jpg

Normal Tissue Figure 4 Soft tissue

Peripheral nerve
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39835/100448_B_1_7.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (HEL, RH-30, SCLC-21H, SH-SY5Y, U-138 MG, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149294-NCAM1

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Specifications

Immunogen
KG-1 human acute myelogenous cell line

Host Species
Mouse

Type
Mouse IgG2a

Specificity
NCAM1

Species Reactivity
Human, Non-human primate

Clone
MEM-188

Applications
IHC, FC

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.4

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
IHC: 1:50, FC: 3 µg/mL

Target

Alternative Names
CD56; NCAM; MSK39;

Gene ID
4684

UniProt ID
P13591

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY104.pdf

MSDS

MSDS - VS-1024-XY104.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Isotype Control

CAT
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Secondary Antibody

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