Mouse Anti-NHP NPTN Recombinant Antibody (clone OTI2D4) (CAT#: VS-1024-XY340)
Designed for versatility, this antibody targets NPTN and demonstrates remarkable reactivity across a variety of species, including dog, human, mouse, and non-human primates. Utilizing full-length human recombinant NPTN protein as the immunogen, it excels in Western Blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC). This mouse-derived antibody serves as a crucial asset for researchers exploring neuronal functions and intercellular interactions, offering insights into cellular dynamics across diverse biological settings. Enhance your studies with this reliable and potent research tool.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/1891_C23_9_cr5b8ea272226ef_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/1891_C23_9_cr5b8ea272226ef_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows distinct positivity in cells in granular layer and neuropil. Purkinje cells were negative.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51497/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows distinct positivity in cells in granular layer and neuropil. Purkinje cells were negative.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51497/ihc_selected.jpg
Figure 3 IHC staining of human endometrium
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_B_3_3_selected.jpg
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_B_3_3_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_7_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156642-NPTN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156642-NPTN
Specifications
- Immunogen
- Full length human recombinant protein of NPTN
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- NPTN
- Species Reactivity
- Dog, Human, Mouse, Non-human primate
- Clone
- OTI2D4
- Applications
- WB, IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, ICC: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3747z | Mouse Anti-NPTN Recombinant Antibody (clone 19H3) | WB, ICC, IF, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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