Mouse Anti-NHP NPTN Recombinant Antibody (clone OTI2D4)
CAT#: VS-1024-XY340
Designed for versatility, this antibody targets NPTN and demonstrates remarkable reactivity across a variety of species, including dog, human, mouse, and non-human primates. Utilizing full-length human recombinant NPTN protein as the immunogen, it excels in Western Blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC). This mouse-derived antibody serves as a crucial asset for researchers exploring neuronal functions and intercellular interactions, offering insights into cellular dynamics across diverse biological settings. Enhance your studies with this reliable and potent research tool.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/1891_C23_9_cr5b8ea272226ef_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows distinct positivity in cells in granular layer and neuropil. Purkinje cells were negative.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51497/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_B_3_3_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72453/159490_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156642-NPTN
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Specifications
- Immunogen
- Full length human recombinant protein of NPTN
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- NPTN
- Species Reactivity
- Dog, Human, Mouse, Non-human primate
- Clone
- OTI2D4
- Applications
- WB, IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, ICC: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NPTN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3747z | Mouse Anti-NPTN Recombinant Antibody (clone 19H3) | WB, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4932 | Anti-NPTN Immunohistochemistry Kit | IHC |
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