Mouse Anti-NHP PAK3 Recombinant Antibody (clone 4G8F11) (CAT#: VS-1024-XY364)
This antibody, directed against PAK3, is engineered from a purified recombinant fragment of human PAK3, ensuring high specificity and relevance. With demonstrated reactivity to both human and non-human primate samples, it is ideally suited for applications in Western Blotting (WB) and Enzyme-Linked Immunosorbent Assay (ELISA). Its precision enables researchers to delve into the role of PAK3 in signaling pathways and cellular dynamics. This versatile tool is instrumental for those seeking to uncover the complexities of cytoskeletal regulation and its implications in various physiological processes.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HAP1
(Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
(Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
(Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
Figure 4 Cerebellum
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HAP1, HDLM-2, NTERA-2, SCLC-21H, SH-SY5Y, U-2197, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
Specifications
- Immunogen
- Purified recombinant fragment of human PAK3
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PAK3
- Species Reactivity
- Human, Non-human primate
- Clone
- 4G8F11
- Applications
- WB, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, ELISA: 1:8000
Target
- Alternative Names
- P21 (RAC1) Activated Kinase 3; P21 Protein (Cdc42/Rac)-Activated Kinase 3; P21 (CDKN1A)-Activated Kinase 3; Oligophrenin-3; EC 2.7.11.1; Beta-PAK; OPHN3; PAK-3; Serine/Threonine-Protein Kinase PAK 3; Adriamycin Resistance-Associated
- Gene ID
- 5063
- UniProt ID
- O75914
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Bladder Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PAK3"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1537CT | Recombinant Mouse anti-Human PAK3 Monoclonal antibody (EML2324) | ICC/IF, WB | |
| VS3-WK72 | Mouse Anti-PAK3 Recombinant Antibody (clone F11-G8) | WB, ELISA | Mouse IgG1 |
| VS3-FY1092 | Recombinant Rabbit Anti-PAK3 (phospho Ser144) Antibody (clone R04-2A4) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR557 | Mouse Anti-PAK3 Recombinant Antibody (clone 4G8A5) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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