Mouse Anti-NHP PAX8 Recombinant Antibody (clone 1F8-3A8)
CAT#: VS-1024-XY368
This mouse-derived antibody targets PAX8, demonstrating significant reactivity with human, mouse, and non-human primate samples. The immunogen, a purified internal fragment of human recombinant PAX8, ensures high specificity for this crucial transcription factor involved in developmental processes. Its versatile applications in Western Blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC) make it an indispensable asset for researchers investigating the role of PAX8 in tissue differentiation and pathology. Utilize this antibody to deepen your exploration of gene regulation and its implications in various diseases.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30062/297_D7_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64554/1789_E6_5_cr598aeb34d7793_selected.jpg
Normal Tissue
Figure 3 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_A_7_5.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_B_3_5.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_A_8_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (EFO-21, GAMG, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125618-PAX8
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Specifications
- Immunogen
- Purified internal fragment of human recombinant PAX8
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PAX8
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 1F8-3A8
- Applications
- WB, IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:20-1:200, ICC: 1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PAX8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0940CL | Mouse Anti-PAX8 Recombinant Antibody (TAB-0940CL) | WB, IHC | Mouse IgG1, κ |
| TAB-0940CL-S(P) | Mouse Anti-PAX8 Recombinant Antibody; scFv Fragment (TAB-0940CL-S(P)) | ELISA, WB, IHC | Mouse scFv |
| TAB-0940CL-F(E) | Mouse Anti-PAX8 Recombinant Antibody; Fab Fragment (TAB-0940CL-F(E)) | ELISA, WB, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2604 | Hi-Affi™ Recombinant Rabbit Anti-PAX8 Monoclonal Antibody (DS2604AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0357C | Mouse Anti-PAX8 Recombinant Antibody (ZG-0357C) | WB, ELISA | Mouse IgG |
| ZG-0399U | Rabbit Anti-PAX8 Recombinant Antibody (clone C25-Q) | ELISA, WB | Rabbit IgG |
| VS3-XY1215 | Mouse Anti-PAX8 Recombinant Antibody (clone 2D4) | ELISA, WB | Mouse IgG1 |
| VS3-XY1216 | Mouse Anti-PAX8 Recombinant Antibody (clone 2B11) | WB | |
| VS3-XY1217 | Mouse Anti-PAX8 Recombinant Antibody (clone 5B1) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC152 | AbPlus™ Anti-PAX8 Magnetic Beads (VS-0724-YC152) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1571 | Anti-PAX8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5212 | Anti-Mouse PAX8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5213 | Anti-Human PAX8 Immunohistochemistry Kit | IHC |
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