Mouse Anti-NHP PAX8 Recombinant Antibody (clone 1F8-3A8) (CAT#: VS-1024-XY368)
This mouse-derived antibody targets PAX8, demonstrating significant reactivity with human, mouse, and non-human primate samples. The immunogen, a purified internal fragment of human recombinant PAX8, ensures high specificity for this crucial transcription factor involved in developmental processes. Its versatile applications in Western Blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC) make it an indispensable asset for researchers investigating the role of PAX8 in tissue differentiation and pathology. Utilize this antibody to deepen your exploration of gene regulation and its implications in various diseases.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30062/297_D7_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30062/297_D7_1_selected.jpg
Figure 2 IF staining of human cell line RPTEC TERT1
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64554/1789_E6_5_cr598aeb34d7793_selected.jpg
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64554/1789_E6_5_cr598aeb34d7793_selected.jpg
Figure 3 Kidney
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_A_7_5.jpg
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_A_7_5.jpg
Figure 4 Thyroid gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_B_3_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_B_3_5.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61888/136405_A_8_4.jpg
Figure 6 RNA cell line category: Group enriched (EFO-21, GAMG, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125618-PAX8
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125618-PAX8
Specifications
- Immunogen
- Purified internal fragment of human recombinant PAX8
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PAX8
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 1F8-3A8
- Applications
- WB, IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:20-1:200, ICC: 1:50-1:200
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0940CL | Mouse Anti-PAX8 Recombinant Antibody (TAB-0940CL) | WB, IHC | Mouse IgG1, κ |
| TAB-0940CL-S(P) | Mouse Anti-PAX8 Recombinant Antibody; scFv Fragment (TAB-0940CL-S(P)) | ELISA, WB, IHC | Mouse scFv |
| TAB-0940CL-F(E) | Mouse Anti-PAX8 Recombinant Antibody; Fab Fragment (TAB-0940CL-F(E)) | ELISA, WB, IHC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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