Mouse Anti-NHP RAD51B Recombinant Antibody (clone 1 H3/13)
CAT#: VS-1024-XY432
Aimed at the detection of RAD51B, this mouse monoclonal antibody exhibits reactivity with human and non-human primate samples. The immunogen is a His-tagged human RAD51B, ensuring robust specificity for accurate detection. Designed for use in Western blot (WB) applications, this antibody serves as a critical reagent for studying RAD51B expression and function, providing reliable insights into its role in cellular processes and enhancing research efforts across various biological investigations.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51869/767_A8_3_selected.jpg
Normal Tissue
Figure 2 Epididymis.
High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_A_1_6_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16191/38158_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182185-RAD51B
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Specifications
- Immunogen
- His-tagged human RAD51B
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- RAD51B
- Species Reactivity
- Human, Non-human primate
- Clone
- 1 H3/13
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
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- Product Name
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Secondary Antibody
- CAT
- Product Name
See other products for "RAD51B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1761CT | Recombinant Mouse anti-Human RAD51B Monoclonal antibody (SBE62C 2F22/7) | WB | |
| FAMAB-1238CQ | Mouse Anti-RAD51B Recombinant Antibody (clone 1h3/13) | IP, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2979 | Hi-Affi™ Recombinant Rabbit Anti-RAD51B Monoclonal Antibody (DS2979AB) | WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC718 | Recombinant Anti-RAD51B Vesicular Antibody, EV Displayed (VS-0425-YC718) | ELISA, FC, Cell-uptake |
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