Mouse Anti-NHP SEMA4D Recombinant Antibody (clone A8)
CAT#: VS-1024-XY70
This monoclonal antibody, originating from mouse, is designed to target SEMA4D with confirmed reactivity in human, mouse, and non-human primate species. Developed using human peripheral blood lymphocytes as the immunogen, it offers high specificity for flow cytometry (FC) and immunofluorescence (IF) applications. Its precise targeting of SEMA4D enables detailed analysis of cell signaling and immune responses, making it an indispensable tool for researchers focused on these pathways across multiple species.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23277/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HDLM-2, HMC-1, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187764-SEMA4D
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Specifications
- Immunogen
- Human peripheral blood lymphocytes
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- SEMA4D
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- A8
- Applications
- IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IF: 1-2 µg/mL, FC: 1-2 µg/106 cells
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SEMA4D"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-559CL | Human Anti-SEMA4D Recombinant Antibody (TAB-559CL) | ELISA | Human IgG |
| TAB-429CT-S(P) | Humanized Anti-SEMA4D Recombinant Antibody; scFv Fragment (TAB-429CT-S(P)) | WB, ELISA, FC, BL | Humanized scFv |
| TAB-430CT-S(P) | Humanized Anti-SEMA4D Recombinant Antibody; scFv Fragment (TAB-430CT-S(P)) | WB, ELISA, FC, BL | Humanized scFv |
| TAB-429CT-F(E) | Humanized Anti-SEMA4D Recombinant Antibody; Fab Fragment (TAB-429CT-F(E)) | WB, ELISA, FC, BL | Humanized Fab |
| TAB-430CT-F(E) | Humanized Anti-SEMA4D Recombinant Antibody; Fab Fragment (TAB-430CT-F(E)) | WB, ELISA, FC, BL | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3206 | Hi-Affi™ Recombinant Rabbit Anti-SEMA4D Monoclonal Antibody (DS3206AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0049-WJ | Mouse Anti-SEMA4D Recombinant Antibody (HPAB-0049-WJ) | FC, ELISA, IHC, FuncS, Inhib | Mouse IgG1 |
| HPAB-AP440-YC | Mouse Anti-SEMA4D Recombinant Antibody (HPAB-AP440-YC) | ELISA, Block, Neut, FuncS | Mouse IgG2b |
| HPAB-0934WJ | Mouse Anti-SEMA4D Recombinant Antibody (HPAB-0934WJ) | Neut | Mouse IgG1 |
| HPAB-0935WJ | Human Anti-SEMA4D Recombinant Antibody (HPAB-0935WJ) | Neut, ELISA | Humanized IgG1 |
| ZG-0137U | Human Anti-SEMA4D Recombinant Antibody (clone 21E8) | ELISA, FC | Humanized IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0049-WJ-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-0049-WJ-F(E)) | FC, ELISA | Mouse Fab |
| HPAB-AP440-YC-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-AP440-YC-F(E)) | ELISA, Block, Neut, FuncS | Mouse Fab |
| HPAB-AP442-YC-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-AP442-YC-F(E)) | ELISA, Block, Neut, FuncS | Mouse Fab |
| HPAB-0934WJ-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-0934WJ-F(E)) | Neut | Mouse Fab |
| HPAB-0935WJ-F(E) | Human Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-0935WJ-F(E)) | Neut, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP440-YC-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-AP440-YC-S(P)) | ELISA, Block, Neut, FuncS | Mouse scFv |
| HPAB-AP442-YC-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-AP442-YC-S(P)) | ELISA, Block, Neut, FuncS | Mouse scFv |
| HPAB-AP443-YC-S(P) | Human Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-AP443-YC-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-0934WJ-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-0934WJ-S(P)) | Neut | Mouse scFv |
| HPAB-0935WJ-S(P) | Human Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-0935WJ-S(P)) | Neut, ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY197 | CytoStream™ Rat Anti-SEMA4D Recombinant Antibody (clone BMA-12) | FC | Rat IgG2a, lambda |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC533 | Recombinant Anti-SEMA4D Vesicular Antibody, EV Displayed (VS-0425-YC533) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6444 | Anti-SEMA4D Immunohistochemistry Kit | IHC |
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