Mouse Anti-NHP SMAD2 Recombinant Antibody (VS-1024-XY484)
CAT#: VS-1024-XY484
Targeting SMAD2, this antibody demonstrates outstanding reactivity with human, mouse, non-human primate, and rat species. The immunogen consists of a recombinant protein specific to the amino terminus of the human SMAD2 protein, facilitating precise recognition and binding. Its primary application in Western blotting allows for effective analysis of protein expression and signaling pathways. Researchers focusing on developmental biology and signal transduction will find this antibody an essential tool, delivering consistent and reliable results across diverse experimental settings.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
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Specifications
- Immunogen
- Recombinant protein specific to the amino terminus of human SMAD2 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SMAD2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800
Target
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Related Diseases
Colorectal Cancer
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
- CAT
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See other products for "SMAD2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3321 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (DS3321AB) | FC, ICC, IHC-P, IP, WB | IgG |
| MOR-4512 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH20DS) | WB, IF, ICC | IgG |
| MOR-4709 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH223DS) | WB, IF, ICC, FC, ChIP | IgG |
| MOR-4735 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH249DS) | WB, IF, ICC, FC, ChIP, ELISA | IgG |
| MOR-0037-FY | Rabbit Anti-SMAD2 Recombinant Antibody (clone AFY0008) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2378-CN | Recombinant Rabbit Anti-SMAD2 (phosphorylated Ser250) Monoclonal Antibody (CBACN-664) | WB, FC | Rabbit IgG |
| MRO-2379-CN | Recombinant Rabbit Anti-SMAD2 (phosphorylated Ser255) Monoclonal Antibody (JF0882) | WB, IHC, IP | Rabbit IgG |
| VS3-QX1018 | Mouse Anti-SMAD2 Recombinant Antibody (clone 4D3) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-QX1019 | Mouse Anti-SMAD2 Recombinant Antibody (clone 6H5-E3-C11) | WB, ICC | Mouse IgG1 |
| VS3-QX1020 | Mouse Anti-SMAD2 Recombinant Antibody (clone 5G7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY238 | AbPlus™ Anti-SMAD2 Magnetic Beads (CBACN-511) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY71 | Human Anti-SMAD2 scFv-Fc Chimera (VS-0125-FY71) | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1077 | CytoStream™ Rabbit Anti-SMAD2 Recombinant Antibody (VS13-YC1077) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2105 | Anti-SMAD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6716 | Anti-Human SMAD2 Immunohistochemistry Kit | IHC |
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