Mouse Anti-NHP SMAD2 Recombinant Antibody (VS-1024-XY484) (CAT#: VS-1024-XY484)
Targeting SMAD2, this antibody demonstrates outstanding reactivity with human, mouse, non-human primate, and rat species. The immunogen consists of a recombinant protein specific to the amino terminus of the human SMAD2 protein, facilitating precise recognition and binding. Its primary application in Western blotting allows for effective analysis of protein expression and signaling pathways. Researchers focusing on developmental biology and signal transduction will find this antibody an essential tool, delivering consistent and reliable results across diverse experimental settings.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
Specifications
- Immunogen
- Recombinant protein specific to the amino terminus of human SMAD2 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SMAD2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800
Target
- Alternative Names
- SMAD Family Member 2; MAD Homolog 2; HSMAD2; JV18-1; HMAD-2; MADH2; MADR2; MAD; Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD; Mothers Against DPP Homolog 2 (Drosophila); Mothers Against Decapentaplegic Homolog 2
- Gene ID
- 4087
- UniProt ID
- Q15796
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Colorectal Cancer
Gastric Cancer
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4052z | Mouse Anti-SMAD2 Recombinant Antibody (clone 30G7) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1885CT | Recombinant Mouse anti-Human SMAD2 Monoclonal antibody (EML538) | IHC-P, WB | |
| MRO-1402-CN | Recombinant Mouse Anti-SMAD2 Monoclonal Antibody (C9-B0) | WB, IHC, IF | Mouse IgG1 |
| MRO-1403-CN | Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (CBACN-511) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1357 | Recombinant Rabbit Anti-SMAD2 (phospho Ser250) Antibody (clone R02-0D1) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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