Rabbit Anti-NHP SMAD3 Recombinant Antibody (clone E.980.9) (CAT#: VS-1024-XY485)
Focused on the SMAD3 target, this antibody demonstrates excellent reactivity with human, mouse, non-human primate, and rat samples. Utilizing a synthetic peptide corresponding to the SMAD3 protein as the immunogen, it ensures precise binding and detection. Its versatility is highlighted through applications such as Western blotting, immunofluorescence, flow cytometry, and immunoprecipitation. Researchers exploring signaling pathways and transcriptional regulation involving SMAD3 will appreciate this antibody's reliable performance, making it an essential component in advancing their investigations into cellular mechanisms.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
Figure 3 IHC staining of human parathyroid gland
(Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
(Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
Figure 4 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3
Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- SMAD3
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- E.980.9
- Applications
- WB, IF, FC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IF: 1:100-1:200, FC: 1:100, IP: 1:100
Target
- Alternative Names
- LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436
- Gene ID
- 4088
- UniProt ID
- P84022
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1886CT | Recombinant Mouse anti-Human SMAD3 Monoclonal antibody (EML539) | IHC-P, WB | |
| VS3-QX1021 | Mouse Anti-SMAD3 Recombinant Antibody (clone 2A1-8F2-8B8) | IHC | Mouse IgG1 |
| VS3-QX1022 | Mouse Anti-SMAD3 Recombinant Antibody (clone 2F3-5E1-6A4) | IHC | Mouse IgG1 |
| VS3-QX1023 | Mouse Anti-SMAD3 Recombinant Antibody (clone 4H4-10A10-7B4) | WB, IHC | Mouse IgG1 |
| VS3-QX1024 | Mouse Anti-SMAD3 Recombinant Antibody (clone 7H6-4E5-4H2) | IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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