Mouse Anti-NHP SMN1 Recombinant Antibody (clone 2F1)
CAT#: VS-1024-XY486
Designed for the detection of SMN1, this mouse monoclonal antibody demonstrates reactivity with human and non-human primate tissues. The immunogen is a purified recombinant fragment of human SMN1, ensuring exceptional specificity. Versatile in application, it is suitable for Western blot (WB), immunohistochemistry (IHC), flow cytometry (FC), and ELISA. This antibody serves as a crucial tool for investigating SMN1 expression and its role in spinal muscular atrophy, facilitating comprehensive research across various experimental settings.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16324/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69888/151521_A_4_4_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73601/1729_C10_3_selected.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_6_6.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_8_3.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_8_8.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_A_9_5.jpg
Normal Tissue
Figure 8 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45271/107128_B_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (MOLT-4, NB-4, T-47d, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172062-SMN1
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Specifications
- Immunogen
- Purified recombinant fragment of human SMN1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SMN1
- Species Reactivity
- Human, Non-human primate
- Clone
- 2F1
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 2F1"
- CAT
- Product Name
See other products for "SMN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3342 | Hi-Affi™ Recombinant Rabbit Anti-SMN1 Monoclonal Antibody (DS3342AB) | WB, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-100R | Mouse Anti-SMN1 Recombinant Antibody (ZG-100R) | WB, ELISA, IHC, IF | Mouse IgG |
| VS7-0425-WR891 | Mouse Anti-SMN1 Monoclonal Antibody (clone 2F1) | WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR892 | Mouse Anti-SMN1 Monoclonal Antibody (clone 5H1) | WB, IHC, ICC | Mouse IgG1 |
| VS7-0425-WR893 | Mouse Anti-SMN1 Monoclonal Antibody (VS7-0425-WR893) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| VS7-0425-WR894 | Mouse Anti-SMN1 Monoclonal Antibody (VS7-0425-WR894) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6750 | Anti-SMN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6751 | Anti-Mouse SMN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6752 | Anti-Rat SMN1 Immunohistochemistry Kit | IHC |
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