Mouse Anti-NHP TNPO1 Recombinant Antibody (clone D45)
CAT#: VS-1024-XY507
Targeting TNPO1, this monoclonal antibody demonstrates robust reactivity across human, mouse, non-human primate, and rabbit samples. It is well-suited for a variety of applications, including Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. Its performance is anchored by a rigorous validation process, ensuring high sensitivity and specificity in detecting TNPO1 expression. This antibody is an invaluable tool for elucidating the functional dynamics of TNPO1 in cellular transport mechanisms and its implications in disease states, providing critical insights for advancing research in molecular biology and therapeutic development.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/38518_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/38518_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/38518_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/38518_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/38518_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16325/38518_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000083312-TNPO1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- TNPO1
- Species Reactivity
- Human, Mouse, Non-human primate, Rabbit
- Clone
- D45
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1 µg/mL, IF: 5 µg/mL, FC: 1.0 µg/106 cells
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone D45"
- CAT
- Product Name
See other products for "TNPO1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2447z | Mouse Anti-TNPO1 Recombinant Antibody (clone 36G12) | WB, ELISA, FC, ICC, IF, IHC, IP | Mouse IgG1 |
| MOB-1822CT | Recombinant Mouse anti-Human TNPO1 Monoclonal antibody (E56) | ELISA, ICC, IF, IHC-P, IP, WB | |
| VS1-YJ217 | Mouse Anti-TNPO1 Recombinant Antibody (clone D45) | ELISA, IF, IHC-P, WB, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2213CQ | Mouse Anti-TNPO1 Recombinant Antibody (NEUT-2213CQ) | ELISA, Neut, WB | Mouse IgG1 |
| NEUT-2214CQ | Mouse Anti-TNPO1 Recombinant Antibody (clone CBL912) | ELISA, Neut, WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7411 | Anti-Mouse TNPO1 Immunohistochemistry Kit | IHC |
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