Mouse Anti-NHP UBE2C Recombinant Antibody (clone 1F5D3) (CAT#: VS-1024-XY524)
Targeting UBE2C, this antibody is generated from a recombinant full-length protein, ensuring high specificity and reactivity with dog, human, mouse, non-human primate, and rat samples. Its applications in Western Blotting (WB) and Immunocytochemistry (ICC) enable detailed investigations into the role of UBE2C in ubiquitination and cell cycle regulation. Recognized for its sensitivity and reliability, this antibody is an essential tool for researchers seeking to elucidate the molecular mechanisms governing protein degradation and their implications in various diseases, including cancer.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
Figure 4 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
Figure 7 Tonsil
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
Figure 8 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
Figure 9 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
Figure 10 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
Specifications
- Immunogen
- Recombinant full length protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- UBE2C
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- 1F5D3
- Applications
- WB, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.5-2 µg/mL, ICC: 2.5-10 µg/mL
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 C; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme C; E2 Ubiquitin-Conjugating Enzyme C; Ubiquitin-Protein Ligase C; UBCH10; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Cyclin-Selective Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2 C
- Gene ID
- 11065
- UniProt ID
- O00762
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1197MZ | Recombinant Mouse Anti-Human UBE2C Antibody | IHC-P, WB | Mouse antibody |
| VS3-QX1211 | Mouse Anti-UBE2C Recombinant Antibody (clone 5D-3F) | WB, IHC | Mouse IgG2a |
| VS3-CJ117 | Mouse Anti-UBE2C Recombinant Antibody (VS3-CJ117) | WB | Mouse IgG1, κ |
| VS3-XY1575 | Mouse Anti-UBE2C Recombinant Antibody (clone 1F5D3) | ELISA, WB, IHC | Mouse IgG2a |
| VS3-FY1511 | Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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