Mouse Anti-NHP UBE2C Recombinant Antibody (clone 1F5D3)
CAT#: VS-1024-XY524
Targeting UBE2C, this antibody is generated from a recombinant full-length protein, ensuring high specificity and reactivity with dog, human, mouse, non-human primate, and rat samples. Its applications in Western Blotting (WB) and Immunocytochemistry (ICC) enable detailed investigations into the role of UBE2C in ubiquitination and cell cycle regulation. Recognized for its sensitivity and reliability, this antibody is an essential tool for researchers seeking to elucidate the molecular mechanisms governing protein degradation and their implications in various diseases, including cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
Normal Tissue
Figure 7 Tonsil
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
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Specifications
- Immunogen
- Recombinant full length protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- UBE2C
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- 1F5D3
- Applications
- WB, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.5-2 µg/mL, ICC: 2.5-10 µg/mL
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 C; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme C; E2 Ubiquitin-Conjugating Enzyme C; Ubiquitin-Protein Ligase C; UBCH10; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Cyclin-Selective Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2 C
- Gene ID
- 11065
- UniProt ID
- O00762
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- CAT
- Product Name
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- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 1F5D3"
- CAT
- Product Name
See other products for "UBE2C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3665z | Mouse Anti-UBE2C Recombinant Antibody (clone 16G12) | WB, IHC, IF, ELISA | Mouse IgG1 |
| MOB-1197MZ | Recombinant Mouse Anti-Human UBE2C Antibody | IHC-P, WB | Mouse antibody |
| FAMAB-1285CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41C-6) | Immuno-MRM, WB | Mouse IgG1 |
| FAMAB-1286CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41A-1) | Immuno-MRM, WB, ELISA | Mouse IgG1 |
| VS3-QX1211 | Mouse Anti-UBE2C Recombinant Antibody (clone 5D-3F) | WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3749 | Rabbit Anti-UBE2C Recombinant Antibody (clone DS3749AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0057 | APC-B*15:02/Human Ubiquitin-conjugating enzyme E2C (ELWKNPTAF) MHC Tetramer | FCM | |
| MHC-CN0058 | PE-B*15:02/Human Ubiquitin-conjugating enzyme E2C (ELWKNPTAF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2415 | Anti-UBE2C Immunohistochemistry Kit | IHC | |
| VS-0525-XY7691 | Anti-Rat UBE2C Immunohistochemistry Kit | IHC | |
| VS-0525-XY7690 | Anti-Human UBE2C Immunohistochemistry Kit | IHC |
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