Rabbit Anti-NKX2-2 Recombinant Antibody (clone R05-5E5)
CAT#: VS3-XY3447
This antibody is a recombinant rabbit antibody targeting the NKX2-2 (NK2 homeobox 2), which may also be involved in specifying diencephalic neuromeric boundaries. It reacts with human, mouse and rat NKX2-2. It has been reported for use in Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Immunohistochemistry-Paraffin and Immunofluorescence is 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/11006_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_9_3.jpg
Normal Tissue
Figure 3 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_1_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HAP1, T-47d, U-138 MG, U-2 OS, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125820-NKX2-2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NKX2-2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-5E5
- Applications
- IHC-P, ICC, IF
- Related Disease
- Oligodendroglioma; Extraosseous Ewing Sarcoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- NKX2B; NKX2.2
- Gene ID
- 4821
- UniProt ID
- O95096
- Long Name
- NK2 homeobox 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O95096)
Modification sites at neXtProt (NX_O95096)
- Protein Refseq
- XP_054179432.1
- Function
- Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity).
May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Binds to elements within the NEUROD1 promoter.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NKX2-2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1725MZ | Recombinant Mouse Anti-Human NKX2-2 Antibody (clone QS-2D22) | WB | Mouse antibody |
| VS3-QX819 | Mouse Anti-NKX2-2 Recombinant Antibody (clone 1C3-C8-F8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK157 | Mouse Anti-NKX2-2 Recombinant Antibody (clone H12-E4) | WB, ELISA | Mouse IgG1 |
| VS13-YC812 | Rabbit Anti-NKX2-2 Recombinant Antibody (VS13-YC812) | IHC-P | Rabbit IgG |
| VS7-0425-WR482 | Mouse Anti-NKX2-2 Recombinant Antibody (clone 3E4) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4854 | Anti-NKX2-2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4855 | Anti-Mouse NKX2-2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4856 | Anti-Chicken NKX2-2 Immunohistochemistry Kit | IHC |
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