Rabbit Anti-NKX2-2 Recombinant Antibody (clone R05-5E5) (CAT#: VS3-XY3447)
This antibody is a recombinant rabbit antibody targeting the NKX2-2 (NK2 homeobox 2), which may also be involved in specifying diencephalic neuromeric boundaries. It reacts with human, mouse and rat NKX2-2. It has been reported for use in Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Immunohistochemistry-Paraffin and Immunofluorescence is 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/11006_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/11006_B_7_5.jpg
Figure 2 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_9_3.jpg
Figure 3 Pancreas
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_1_3.jpg
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_1_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_6_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3468/10935_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (HAP1, T-47d, U-138 MG, U-2 OS, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125820-NKX2-2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125820-NKX2-2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NKX2-2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-5E5
- Applications
- IHC-P, ICC, IF
- Related Disease
- Oligodendroglioma; Extraosseous Ewing Sarcoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- NKX2B; NKX2.2
- Gene ID
- 4821
- UniProt ID
- O95096
- Long Name
- NK2 homeobox 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O95096)
Modification sites at neXtProt (NX_O95096)
- Protein Refseq
- XP_054179432.1
- Function
- Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity).
May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Binds to elements within the NEUROD1 promoter.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1725MZ | Recombinant Mouse Anti-Human NKX2-2 Antibody (clone QS-2D22) | WB | Mouse antibody |
| VS3-QX819 | Mouse Anti-NKX2-2 Recombinant Antibody (clone 1C3-C8-F8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK157 | Mouse Anti-NKX2-2 Recombinant Antibody (clone H12-E4) | WB, ELISA | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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