Rabbit Anti-NONO Recombinant Antibody (VS13-YC824)
CAT#: VS13-YC824
The Rabbit Anti-NONO Recombinant Antibody is specific for human, mouse and rat NONO. NONO, an RNA-binding protein, plays roles in transcriptional regulation and splicing, with associations to renal carcinomas and alternative transcripts known. The anti-NONO antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/877_A7_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity and additional strong cytoplasmic staining.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22069/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_6_6.jpg
Normal Tissue
Figure 8 Ovary
Follicle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_5_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147140-NONO
❮
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Specifications
- Immunogen
- Recombinant human NMT55/p54nrb.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NONO
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- Non-POU Domain Containing Octamer Binding; Protein Phosphatase 1, Regulatory Subunit 114; DNA-Binding P52/P100 Complex, 52 KDa Subunit; 54 KDa Nuclear RNA- And DNA-Binding Protein; 55 KDa Nuclear Protein; P54(Nrb); P54NRB; NMT55; NRB54; Non-Pou Domain-Containing Octamer (ATGCAAAT) Binding Protein;
- Gene ID
- 4841
- UniProt ID
- Q15233
- Long Name
- Non-POU Domain Containing Octamer Binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NONO"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3007z | Mouse Anti-NONO Recombinant Antibody (clone 24H3) | WB, IF, IHC | Mouse IgG1 |
| HPAB-0766-CN | Human Anti-NONO Recombinant Antibody (HPAB-0766-CN) | WB, ELISA, IHC, IP | Human IgG |
| MRO-1154-CN | Recombinant Rabbit Anti-NONO Monoclonal Antibody (JG87-38) | WB, IF, IHC | Rabbit IgG |
| ZG-0061U | Rabbit Anti-NONO Recombinant Antibody (clone 8C11) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-FY1048 | Recombinant Rabbit Anti-NONO Antibody (clone R05-1F8) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538MZ | Anti-Human NONO Recombinant Antibody (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538MZ-S(P) | Anti-Human NONO Recombinant Antibody scFv Fragment (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| TAB-538MZ-F(E) | Anti-Human NONO Recombinant Antibody Fab Fragment (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2467 | Hi-Affi™ Recombinant Rabbit Anti-NONO Monoclonal Antibody (DS2467AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0766-CN-S(P) | Human Anti-NONO Recombinant Antibody; scFv Fragment (HPAB-0766-CN-S(P)) | WB, ELISA, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0766-CN-F(E) | Human Anti-NONO Recombinant Antibody; Fab Fragment (HPAB-0766-CN-F(E)) | WB, ELISA, IHC, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4894 | Anti-NONO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4895 | Anti-Mouse NONO Immunohistochemistry Kit | IHC |
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