Recombinant Humanized Anti-Norovirus Antibody (NV1A8) (CAT#: HPAB-2430LY)

Figure 1 Binding and blocking characteristics of purified monoclonal antibodies.

Purified IgG (red) or IgA (blue) antibodies were tested for binding to NV VLP in ELISA (FIG. A) or for blocking VLP-glycan interaction (FIG. B). Each of the IgG antibodies bound to VLPs with lower EC50 values than IgA antibodies, while in contrast the concentrations needed for blocking were similar for IgG and IgA. The blocking of murine mAb 8812 is shown in black.

Figure 2 Specificity of human mAbs.

The binding (mean absorbance at 450 nm + SD) of purified mAbs at 20 μg/mL to VLPs representing homologous virus (NoV GI.I) or heterologous human NoVs of different genotypes (FIG. A) or antigens representing wild-type or mutant recombinant capsid proteins of homologous virus (FIG. B) were assessed by ELISA to evaluate genotype specificity and to infer the subdomain of major capsid protein bound by anti norovirus mAbs. The data shown in each figure summarizes the results from 2 independent experiments.