Rabbit Anti-NOS2 Recombinant Antibody (clone R05-6G2)
CAT#: VS3-XY3449
It is a recombinant rabbit antibody specific for NOS2 (Nitric oxide synthase 2), which involves in inflammation, enhances the synthesis of pro-inflammatory mediators. The antibody specifically reacts with human and mouse NOS2. This product has been reported for use in Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunoprecipitation and Immunofluorescence is 1/500-1/1000, 1/20 and 1/50-1/200, respectively.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2014/166726_B_8_5.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2014/166726_A_9_4.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (CACO-2, SCLC-21H)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000007171-NOS2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse NOS2
- Species Reactivity
- Human, Mouse
- Clone
- R05-6G2
- Applications
- WB, ICC, IF, IP
- Related Disease
- Malaria; Glioblastoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- NOS; INOS; NOS2A; HEP-NOS
- Gene ID
- 4843
- UniProt ID
- P35228
- Long Name
- Nitric oxide synthase 2
- Sequence Similarities
- Belongs to the NOS family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
- Protein Refseq
- NP_000616.3
- Function
- Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004, PubMed:7682706, PubMed:7504305).
In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity).
As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112).
Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NOS2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1473z | Mouse Anti-NOS2 Recombinant Antibody (clone 26B5) | WB, ELISA, FC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-2264MZ | Recombinant Mouse Anti-Mouse NOS2 Antibody (clone OPT-JO) | ICC, IF, WB | Mouse antibody |
| MRO-1976-CN | Rabbit Anti-NOS2 Polyclonal Antibody (MRO-1976-CN) | IF, IHC, FC, WB | Rabbit IgG |
| VS3-XY1171 | Mouse Anti-NOS2 Recombinant Antibody (clone 4E5) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS4-CJ56 | Recombinant Mouse Anti-NOS2 Antibody (clone 2H6B10) | ELISA, WB, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2472 | Hi-Affi™ Recombinant Rabbit Anti-NOS2 Monoclonal Antibody (DS2472AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-044CN | Human Anti-NOS2 Recombinant Antibody (clone 1E8-B8) | ELISA, FC | Human IgG1, κ |
| NS-045CN | Human Anti-NOS2 Recombinant Antibody (clone 2D10-2H9) | ELISA, FC | Human IgG2a, κ |
| NS-046CN | Human Anti-NOS2 Recombinant Antibody (clone 24H9-1F3) | ELISA, FC | Chimeric (mouse/human) IgG1, κ |
| NS-046CN-F(E) | Human Anti-NOS2 Recombinant Antibody (clone 24H9-1F3); Fab Fragment | ELISA, FC | Chimeric (mouse/human) Fab |
| NS-046CN-S(P) | Mouse Anti-NOS2 Recombinant Antibody (clone 24H9-1F3); scFv Fragment | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-044CN-F(E) | Human Anti-NOS2 Recombinant Antibody (clone 1E8-B8); Fab Fragment | ELISA, FC | Human Fab |
| NS-045CN-F(E) | Human Anti-NOS2 Recombinant Antibody (clone 2D10-2H9); Fab Fragment | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-044CN-S(P) | Human Anti-NOS2 Recombinant Antibody (clone 1E8-B8); scFv Fragment | ELISA, FC | Human scFv |
| NS-045CN-S(P) | Human Anti-NOS2 Recombinant Antibody (clone 2D10-2H9); scFv Fragment | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4900 | Anti-NOS2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4901 | Anti-Mouse NOS2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4902 | Anti-Bovine NOS2 Immunohistochemistry Kit | IHC |
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