Rabbit Anti-NOS2 Recombinant Antibody (clone R05-6G2) (CAT#: VS3-XY3449)
It is a recombinant rabbit antibody specific for NOS2 (Nitric oxide synthase 2), which involves in inflammation, enhances the synthesis of pro-inflammatory mediators. The antibody specifically reacts with human and mouse NOS2. This product has been reported for use in Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunoprecipitation and Immunofluorescence is 1/500-1/1000, 1/20 and 1/50-1/200, respectively.
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2014/166726_B_8_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2014/166726_B_8_5.jpg
Figure 2 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2014/166726_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2014/166726_A_9_4.jpg
Figure 3 RNA cell line category: Group enriched (CACO-2, SCLC-21H)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000007171-NOS2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000007171-NOS2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse NOS2
- Species Reactivity
- Human, Mouse
- Clone
- R05-6G2
- Applications
- WB, ICC, IF, IP
- Related Disease
- Malaria; Glioblastoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- NOS; INOS; NOS2A; HEP-NOS
- Gene ID
- 4843
- UniProt ID
- P35228
- Long Name
- Nitric oxide synthase 2
- Sequence Similarities
- Belongs to the NOS family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
- Protein Refseq
- NP_000616.3
- Function
- Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004, PubMed:7682706, PubMed:7504305).
In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity).
As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112).
Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "NOS2"
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IgG Antibody Products
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1473z | Mouse Anti-NOS2 Recombinant Antibody (clone 26B5) | WB, ELISA, FC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-2264MZ | Recombinant Mouse Anti-Mouse NOS2 Antibody (clone OPT-JO) | ICC/IF, WB | Mouse antibody |
| MRO-1976-CN | Rabbit Anti-NOS2 Polyclonal Antibody (MRO-1976-CN) | IF, IHC, FC, WB | Rabbit IgG |
| VS3-XY1171 | Mouse Anti-NOS2 Recombinant Antibody (clone 4E5) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS4-CJ56 | Recombinant Mouse Anti-NOS2 Antibody (clone 2H6B10) | ELISA, WB, FC | Mouse IgG2b |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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