Anti-NOTCH1 Antibody Prodrug, Protease Activated (OMP-52M51)
CAT#: VS-1025-YC28
The Anti-NOTCH1 Antibody Prodrug, Protease Activated (OMP-52M51) is an antibody prodrug comprised of a recombinant mouse antibody targeting human NOTCH1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to NOTCH1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to NOTCH1 effectively. NOTCH1 is a transmembrane receptor that acts as both an oncogene and a tumor suppressor, depending on the cellular context. Activating mutations are a primary driver in over 50% of T-cell Acute Lymphoblastic Leukemia (T-ALL), where it promotes proliferation and survival. Conversely, its function can be lost in certain solid tumors, allowing uncontrolled growth.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67168/1315_F7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22466/61564_A_3_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HaCaT, HDLM-2, HMC-1, MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148400-NOTCH1
❮
❯
Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human NOTCH1
- Target
- NOTCH1
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- OMP-52M51
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
- Alternative Names
- hN1; AOS5; TAN1; AOVD1
- Gene ID
- 4851
- UniProt ID
- P46531
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Related Diseases
Breast Cancer
Endocrine Resistance
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone OMP-52M51"
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See other products for "NOTCH1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1747z | Mouse Anti-NOTCH1 Recombinant Antibody (clone 21H9) | WB, ICC, IF, IHC | Mouse IgG2b |
| PABL-666 | Human Anti-NOTCH1 Recombinant Antibody | ELISA | Human IgG |
| MOB-1438CT | Recombinant Mouse anti-Mouse NOTCH1 Monoclonal antibody (3E12) | FC | |
| HPAB-0078-YC | Mouse Anti-NOTCH1 Recombinant Antibody (clone OMP-52M51) | IHC | Mouse IgG |
| HPAB-0044LY | Mouse Anti-NOTCH1 Recombinant Antibody (HPAB-0044LY) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H11 | Anti-Human NOTCH1 Recombinant Antibody (Brontictuzumab) | Inhib | IgG2 - lambda |
| TAB-541MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-541MZ) | Neut, WB | Humanized antibody |
| TAB-542MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-542MZ) | Neut, WB | Humanized antibody |
| TAB-544MZ | Anti-Human NOTCH1 Recombinant Antibody (A12.2) | ELISA, FuncS, Inhib | Humanized antibody |
| TAB-541MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-541MZ-S(P)) | WB, Competition ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-660 | Human Anti-NOTCH1 Recombinant Antibody; Fab Fragment | ELISA | Human Fab |
| HPAB-0078-YC-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone OMP-52M51); Fab Fragment | IHC | Mouse Fab |
| NS-060CN-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2E6); Fab Fragment | FC, IF, ELISA | Mouse Fab |
| NS-061CN-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2G10); Fab Fragment | FC, IF, ELISA | Mouse Fab |
| NS-062CN-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2A11); Fab Fragment | FC, IF, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-660 | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment | ELISA | Human scFv |
| HPAB-0078-YC-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone OMP-52M51); scFv Fragment | IHC | Mouse scFv |
| NS-060CN-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2E6); scFv Fragment | FC, IF, ELISA | Mouse scFv |
| NS-061CN-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2G10); scFv Fragment | FC, IF, ELISA | Mouse scFv |
| NS-062CN-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2A11); scFv Fragment | FC, IF, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-539MZ | Rat Anti-NOTCH1 Recombinant Antibody (TAB-539MZ) | WB, Competition ELISA, Neut | Rat IgG1 |
| TAB-540MZ | Rat Anti-NOTCH1 Recombinant Antibody (TAB-540MZ) | WB, Competition ELISA, Neut | Rat IgG1 |
| TAB-543MZ | Anti-Human NOTCH1 Recombinant Antibody (mAb N248A) | ELISA, WB, Luciferase reporter assay | |
| TAB-539MZ-S(P) | Rat Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-539MZ-S(P)) | WB, Competition ELISA | Rat scFv |
| TAB-540MZ-S(P) | Rat Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-540MZ-S(P)) | WB, Competition ELISA | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-545MZ | Anti-Human NOTCH1 Recombinant Antibody (23814) | ELISA, FuncS, Inhib, WB, FACS | Human antibody |
| TAB-546MZ | Anti-Human NOTCH1 Recombinant Antibody (23418.8) | FuncS, Inhib | Human antibody |
| TAB-547MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-547MZ) | Inhib | Human IgG1 |
| TAB-548MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-548MZ) | FuncS, Inhib | Human IgG1 |
| TAB-549MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-549MZ) | Inhib | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1786CQ | Recombinant Mouse Anti-NOTCH1 Antibody (MHN1-519) | FC, BL | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2473 | Hi-Affi™ Recombinant Rabbit Anti-NOTCH1 Monoclonal Antibody (DS2473AB) | WB, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-060CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2E6) | FC, IF, ELISA, Inhib, FuncS | Mouse IgG1 |
| NS-061CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2G10) | FC, IF, ELISA | Mouse IgG1 |
| NS-062CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2A11) | FC, IF, ELISA, Inhib | Mouse IgG2b |
| NS-063CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2D11) | FC, IF, ELISA | Mouse IgG1 |
| NS-063CN-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2D11); Fab Fragment | FC, IF, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H11 | Afuco™ Anti-NOTCH1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H11) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC79 | Rabbit Anti-NOTCH1 Antibody (VS-0924-YC79) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY345 | Mouse Anti-NHP NOTCH1 Recombinant Antibody (clone mN1A) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC828 | CytoStream™ Rabbit Anti-NOTCH1 Recombinant Antibody (VS13-YC828) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| VS13-YC829 | CytoStream™ Rabbit Anti-NOTCH1 Polyclonal Antibody (VS13-YC829) | WB, IHC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY159 | Human Anti-NOTCH1 scFv-Fc Chimera (VS-0425-FY159) | ELISA, Inhib, WB, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1506 | Anti-NOTCH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4907 | Anti-Mouse NOTCH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4906 | Anti-Human NOTCH1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC381 | Recombinant Anti-NOTCH1 Vesicular Antibody, EV Displayed (VS-0425-YC381) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC139 | Recombinant Anti-NOTCH1 (LBD domain x HD-LNR domain) Biparatopic Antibody, Tandem scFv (Clone hN1wc49 x Clone N1wc104) | ELISA | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC267 | SmartAb™ Recombinant Anti-NOTCH1 pH-dependent Antibody (Clone Brontictuzumab) | Inhibition | Human IgG2 lambda |
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