Recombinant Anti-NOTCH1 (LBD domain x HD-LNR domain) Biparatopic Antibody, Tandem scFv (Clone hN1wc49 x Clone N1wc104)
CAT#: VS-0525-YC139
The Recombinant Anti-NOTCH1 (LBD domain x HD-LNR domain) Biparatopic Antibody, Tandem scFv (Clone hN1wc49 x Clone N1wc104) is a biparatopic antibody (bpAb) that recognizes two distinct epitopes of NOTCH1. The bpAb's tandem scFv design fuses the hN1wc49 scFv (targeting the ligand binding domain, LBD, of human Notch1) N-terminally to the N1wc104 scFv (specific for the HD-LNR domain of human Notch1).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67168/1315_F7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22466/61564_A_3_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HaCaT, HDLM-2, HMC-1, MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148400-NOTCH1
❮
❯
Specifications
- Host Animal 1
- Human
- Host Animal 2
- Human
- Specificity
- Human NOTCH1
- Species Reactivity
- Human
- Type
- Tandem scFv
- Valency
- 1 + 1
- Clone 1
- hN1wc49
- Clone 2
- N1wc104
- Epitope 1
- Ligand Binding Domain (LBD)
- Epitope 2
- HD-LNR domain
- Purity
- >90%
- Purification
- Affinity purified
- Applications
- ELISA
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid freeze-thaw cycles.
- Long Name
- Notch 1
Target
- Introduction
- Notch 1 is a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
- Alternative Names
- Notch 1; Translocation-Associated Notch Protein TAN-1; TAN1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1, Translocation-Associated (Drosophila); Notch Homolog 1, Translocation-Associated
- Gene ID
- 4851
- UniProt ID
- P46531
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Related Diseases
Breast Cancer
Endocrine Resistance
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-540MZ-S(P) | Rat Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-540MZ-S(P)) | WB, Competition ELISA | Rat scFv |
| TAB-543MZ-S(P) | Anti-Human NOTCH1 Recombinant Antibody scFv Fragment (mAb N248A) | ELISA, WB, Luciferase reporter assay | |
| TAB-539MZ-F(E) | Rat Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-539MZ-F(E)) | WB, Competition ELISA | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-545MZ-S(P) | Anti-Human NOTCH1 Recombinant Antibody scFv Fragment (23814) | ELISA | Human antibody |
| TAB-546MZ-S(P) | Anti-Human NOTCH1 Recombinant Antibody scFv Fragment (23418.8) | ELISA | Human antibody |
| TAB-547MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-547MZ-S(P)) | ELISA | Human scFv |
| TAB-548MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-548MZ-S(P)) | ELISA | Human scFv |
| TAB-549MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-549MZ-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1786CQ | Recombinant Mouse Anti-NOTCH1 Antibody (MHN1-519) | FC, BL | IgG1, κ |
| CAT | Product Name | Application | Type |
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| MOR-2473 | Hi-Affi™ Recombinant Rabbit Anti-NOTCH1 Monoclonal Antibody (DS2473AB) | WB, IHC | IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-060CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2E6) | FC, IF, ELISA, Inhib, FuncS | Mouse IgG1 |
| NS-061CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2G10) | FC, IF, ELISA | Mouse IgG1 |
| NS-062CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2A11) | FC, IF, ELISA, Inhib | Mouse IgG2b |
| NS-063CN-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2D11); Fab Fragment | FC, IF, ELISA | Mouse Fab |
| NS-063CN-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2D11); scFv Fragment | FC, IF, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H11 | Afuco™ Anti-NOTCH1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H11) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0085-YJ | Rat Anti-NOTCH1 Recombinant Antibody (HPAB-0085-YJ) | IF, IHC, WB | Rat IgG1, κ |
| HPAB-0681-WJ | Human Anti-NOTCH1 Recombinant Antibody (clone h52M51) | ELISA, WB | Humanized IgG |
| FAMAB-1388CQ | Mouse Anti-NOTCH1 Recombinant Antibody (clone E6) | Inhib, Block, ELISA, IF | Mouse IgG1 |
| VS3-CJ976 | Rabbit Anti-NOTCH1 Recombinant Antibody (VS3-CJ976) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1049 | Recombinant Rabbit Anti-NOTCH1 Antibody (clone R02-2G9) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP750-YC-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc133); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-AP751-YC-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone hN1wc155); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-AP752-YC-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone hN1wc49); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-AP753-YC-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc104); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-AP754-YC-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc629); scFv Fragment | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS13-YC828 | CytoStream™ Rabbit Anti-NOTCH1 Recombinant Antibody (VS13-YC828) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| VS13-YC829 | CytoStream™ Rabbit Anti-NOTCH1 Polyclonal Antibody (VS13-YC829) | WB, IHC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY4906 | Anti-Human NOTCH1 Immunohistochemistry Kit | IHC |
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