Mouse Anti-NOTCH1 Recombinant Antibody (clone E6) (CAT#: FAMAB-1388CQ)

Figure 1 Cell based assays for identification of blocking antibodies to Notch1 and2.

Six sequence unique anti-NRR1 antibodies were identified to efficiently block activation in HEK-Notch1 cells compared to an unrelated control antibody.

Falk, R., Falk, A., Dyson, M. R., Melidoni, A. N., Parthiban, K., Young, J. L., ... & McCafferty, J. (2012). Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods, 58(1), 69-78.

Figure 2 Specificity of anti-Notch1 and anti-Notch 2 blocking antibodies.

ELISA experiments were carried out with serial dilutions of antibodies on immobilised NRR domains ofmouse Notch1.

Falk, R., Falk, A., Dyson, M. R., Melidoni, A. N., Parthiban, K., Young, J. L., ... & McCafferty, J. (2012). Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods, 58(1), 69-78.

Figure 3 Receptor specific and dose dependent inhibition of Notch signalling between cells.

Dose response curves were generated by titration of N1_E6 on HEK-Notch1 andantibodies N2_B6 and N2_B9 on HEK-Notch2.

Falk, R., Falk, A., Dyson, M. R., Melidoni, A. N., Parthiban, K., Young, J. L., ... & McCafferty, J. (2012). Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods, 58(1), 69-78.

Figure 4 Inhibition of Notch in human NES cells promotes neuronal differentiation.

NES cells were differentiated for 7 days treated with antibody blockade of Notch1-3. Cells were then stained with the post-mitotic neuronal marker beta-III-tubulin (Tuj1) which showed that inhibition of Notch increased the amount of Tuj1 + cells.

Falk, R., Falk, A., Dyson, M. R., Melidoni, A. N., Parthiban, K., Young, J. L., ... & McCafferty, J. (2012). Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods, 58(1), 69-78.