Rabbit Anti-NOTCH1 Recombinant Antibody (clone R01-5B3) (CAT#: VS3-XY3450)
This is a recombinant rabbit antibody that specifically recognizes human NOTCH1 (Notch receptor 1), which affects the implementation of differentiation, proliferation and apoptotic programs. The antibody also has cross-reactivity against mouse or rat NOTCH1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67168/1315_F7_4_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67168/1315_F7_4_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22466/61564_A_3_3_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22466/61564_A_3_3_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_B_8_5.jpg
Figure 4 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_6_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_6_6.jpg
Figure 8 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (HaCaT, HDLM-2, HMC-1, MOLT-4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148400-NOTCH1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148400-NOTCH1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NOTCH1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5B3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Aortic Valve Disease 1; Adams-Oliver Syndrome 5
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- hN1; AOS5; TAN1; AOVD1
- Gene ID
- 4851
- UniProt ID
- P46531
- Long Name
- Notch receptor 1
- Sequence Similarities
- Belongs to the NOTCH family.
- Cellular Localization
- Cell membrane, Membrane, Nucleus
- Post Translation Modifications
- Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form (By similarity).
Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (PubMed:24226769).
- Protein Refseq
- NP_060087.3
- Function
- Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Breast Cancer
Endocrine Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOTCH1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-666 | Human Anti-NOTCH1 Recombinant Antibody | ELISA | Human IgG |
| HPAB-0044LY | Mouse Anti-NOTCH1 Recombinant Antibody (HPAB-0044LY) | WB | Mouse IgG1 |
| MRO-1102-CN | Recombinant Rabbit Anti-NOTCH1 Monoclonal Antibody (CBACN-405) | WB, IF, IHC, IP, FC | Rabbit IgG |
| FN-101CQ | Recombinant Armenian hamster Anti-NOTCH1 Antibody (HMN1-12) | FC, IHC | Armenian Hamster IgG |
| HPAB-0681-WJ | Human Anti-NOTCH1 Recombinant Antibody (clone h52M51) | ELISA, WB | Humanized IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-660 | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment | ELISA | Human scFv |
| HPAB-0078-YC-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone OMP-52M51); scFv Fragment | IHC | Mouse scFv |
| HPAB-0044LY-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody; scFv Fragment (HPAB-0044LY-S(P)) | WB | Mouse scfv |
| HPAB-0681-WJ-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone h52M51); scFv Fragment | ELISA, WB | Humanized scFv |
| HPAB-AP750-YC-S(P) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc133); scFv Fragment | ELISA, FC | Human scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-545MZ | Anti-Human NOTCH1 Recombinant Antibody (23814) | ELISA, FuncS, Inhib, WB, FACS | Human antibody |
| TAB-546MZ | Anti-Human NOTCH1 Recombinant Antibody (23418.8) | FuncS, Inhib | Human antibody |
| TAB-547MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-547MZ) | Inhib | Human IgG1 |
| TAB-548MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-548MZ) | FuncS, Inhib | Human IgG1 |
| TAB-549MZ | Human Anti-NOTCH1 Recombinant Antibody (TAB-549MZ) | Inhib | Human IgG1 |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-539MZ-S(P) | Rat Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-539MZ-S(P)) | WB, Competition ELISA | Rat scFv |
| TAB-540MZ-S(P) | Rat Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-540MZ-S(P)) | WB, Competition ELISA | Rat scFv |
| TAB-543MZ-S(P) | Anti-Human NOTCH1 Recombinant Antibody scFv Fragment (mAb N248A) | ELISA, WB, Luciferase reporter assay | |
| TAB-539MZ-F(E) | Rat Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-539MZ-F(E)) | WB, Competition ELISA | Rat Fab |
| TAB-540MZ-F(E) | Rat Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-540MZ-F(E)) | WB, Competition ELISA | Rat Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-541MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-541MZ-S(P)) | WB, Competition ELISA | Humanized scFv |
| TAB-542MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-542MZ-S(P)) | WB, Competition ELISA | Humanized scFv |
| TAB-544MZ-S(P) | Anti-Human NOTCH1 Recombinant Antibody scFv Fragment (A12.2) | ELISA, WB, Luciferase reporter assay | Humanized antibody |
| TAB-541MZ-F(E) | Human Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-541MZ-F(E)) | WB, Competition ELISA | Humanized Fab |
| TAB-542MZ-F(E) | Human Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-542MZ-F(E)) | WB, Competition ELISA | Humanized Fab |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1786CQ | Recombinant Mouse Anti-NOTCH1 Antibody (MHN1-519) | FC, BL | IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2473 | Hi-Affi™ Recombinant Rabbit Anti-NOTCH1 Monoclonal Antibody (DS2473AB) | WB, IHC | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0078-YC-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone OMP-52M51); Fab Fragment | IHC | Mouse Fab |
| HPAB-AP750-YC-F(E) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc133); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-AP751-YC-F(E) | Human Anti-NOTCH1 Recombinant Antibody (clone hN1wc155); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-AP752-YC-F(E) | Human Anti-NOTCH1 Recombinant Antibody (clone hN1wc49); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-AP753-YC-F(E) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc104); Fab Fragment | ELISA, FC | Human Fab |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-060CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2E6) | FC, IF, ELISA, Inhib, FuncS | Mouse IgG1 |
| NS-061CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2G10) | FC, IF, ELISA | Mouse IgG1 |
| NS-062CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2A11) | FC, IF, ELISA, Inhib | Mouse IgG2b |
| NS-063CN-F(E) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2D11); Fab Fragment | FC, IF, ELISA | Mouse Fab |
| NS-063CN-S(P) | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2D11); scFv Fragment | FC, IF, ELISA | Mouse scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H11 | Afuco™ Anti-NOTCH1 ADCC Recombinant Antibody (Brontictuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
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