Anti-NOTCH3 Antibody Prodrug, Protease Activated (122M23)

Name: Anti-NOTCH3 Antibody Prodrug, Protease Activated (122M23)
SKU: VS-1025-YC94
Rating: 5 (1 reviews)

CAT#: VS-1025-YC94

The Anti-NOTCH3 Antibody Prodrug, Protease Activated (122M23) is an antibody prodrug comprised of a recombinant mouse antibody targeting human NOTCH3, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to NOTCH3 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to NOTCH3 effectively. NOTCH3 is a transmembrane receptor that primarily acts as an oncogene in ovarian cancer and T-cell Acute Lymphoblastic Leukemia (T-ALL), where its signaling drives cell proliferation and survival. However, like other NOTCH family members, its role is complex, and it can occasionally function as a tumor suppressor in other settings.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human rectum

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg

Normal Tissue Figure 6 Liver

Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3

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Specifications

Host Animal
Mouse

Applications
In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay

Specificity
Human NOTCH3

Target
NOTCH3

Species Reactivity
Human

Isotype
IgG

Clone
122M23

Epitope
AA 350-580

Masking Approach
Affinity mask

Mask
A synthetic peptide

Protease Cleavable Linker
MMP-cleavable linker

Protease Substrate
MMPs

Purification
Protein A purified

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Introduction
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Alternative Names
NOTCH3; notch 3; IMF2; CASIL; CADASIL; neurogenic locus notch homolog protein 3; Notch homolog 3

Gene ID
4854

UniProt ID
Q9UM47

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Related Diseases

Breast Cancer

Endocrine Resistance

Downloadable Resources

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Datasheet

Datasheet - VS-1025-YC94.pdf

MSDS

MSDS - VS-1025-YC94.pdf

COA

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