Mouse Anti-Notch3 Recombinant Antibody (clone 2E4D11)

Name: Mouse Anti-Notch3 Recombinant Antibody (clone 2E4D11)
SKU: VS7-0425-WR490
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR490

This product is a mouse IgG2b antibody that binds to human Notch3. The antibody 2E4D11 recognizes specific amino acid domain of human Notch3 (AA: 47-156). It can be used for WB and ELISA assays.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human rectum

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg

Normal Tissue Figure 6 Liver

Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3

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Specifications

Immunogen
Recombinant human Notch3 (AA: 47-156) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG2b

Specificity
Human Notch3

Species Reactivity
Human

Clone
2E4D11

Molecule Class
Transmembrane Receptor

Applications
WB, ELISA

Conjugate
Unconjugated

Related Disease
Cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, 1 (CADASIL1); Myofibromatosis, infantile 2 (IMF2); Lateral meningocele syndrome (LMNS)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
NOTCH3; CASIL; Notch Homolog 3; IMF2; CADASIL; Neurogenic Locus Notch Homolog Protein 3; Notch 3

Gene ID
4854

UniProt ID
Q9UM47

Long Name
Notch Receptor 3

Cellular Localization
Cell membrane; Membrane; Nucleus

Post Translation Modifications
Disulfide bond; Glycoprotein; Methylation; Phosphoprotein

Protein Refseq
NP_000426.2

Function
Activator; Developmental protein; Receptor

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Related Diseases

Breast Cancer

Endocrine Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR490.pdf

MSDS

MSDS - VS7-0425-WR490.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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