Mouse Anti-Notch3 Recombinant Antibody (clone 2E4D11)
CAT#: VS7-0425-WR490
This product is a mouse IgG2b antibody that binds to human Notch3. The antibody 2E4D11 recognizes specific amino acid domain of human Notch3 (AA: 47-156). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
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Specifications
- Immunogen
- Recombinant human Notch3 (AA: 47-156) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human Notch3
- Species Reactivity
- Human
- Clone
- 2E4D11
- Molecule Class
- Transmembrane Receptor
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, 1 (CADASIL1); Myofibromatosis, infantile 2 (IMF2); Lateral meningocele syndrome (LMNS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NOTCH3; CASIL; Notch Homolog 3; IMF2; CADASIL; Neurogenic Locus Notch Homolog Protein 3; Notch 3
- Gene ID
- 4854
- UniProt ID
- Q9UM47
- Long Name
- Notch Receptor 3
- Cellular Localization
- Cell membrane; Membrane; Nucleus
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Methylation; Phosphoprotein
- Protein Refseq
- NP_000426.2
- Function
- Activator; Developmental protein; Receptor
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Related Diseases
Breast Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| TAB-554MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-554MZ-S(P)) | ELISA | Human scFv |
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| HPAB-0283-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-0283-YC-F(E)) | ELISA, IHC, WB | Mouse Fab |
| HPAB-0284-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-0284-YC-F(E)) | ELISA, IHC, WB | Mouse Fab |
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