Mouse Anti-Notch3 Recombinant Antibody (clone 2E4D11) (CAT#: VS7-0425-WR490)
This product is a mouse IgG2b antibody that binds to human Notch3. The antibody 2E4D11 recognizes specific amino acid domain of human Notch3 (AA: 47-156). It can be used for WB and ELISA assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
Figure 6 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
Specifications
- Immunogen
- Recombinant human Notch3 (AA: 47-156) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human Notch3
- Species Reactivity
- Human
- Clone
- 2E4D11
- Molecule Class
- Transmembrane Receptor
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, 1 (CADASIL1); Myofibromatosis, infantile 2 (IMF2); Lateral meningocele syndrome (LMNS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NOTCH3; CASIL; Notch Homolog 3; IMF2; CADASIL; Neurogenic Locus Notch Homolog Protein 3; Notch 3
- Gene ID
- 4854
- UniProt ID
- Q9UM47
- Long Name
- Notch Receptor 3
- Cellular Localization
- Cell membrane; Membrane; Nucleus
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Methylation; Phosphoprotein
- Protein Refseq
- NP_000426.2
- Function
- Activator; Developmental protein; Receptor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
Breast Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1929z | Mouse Anti-NOTCH3 Recombinant Antibody (clone 22H2) | WB, ELISA | Mouse IgG2a |
| HPAB-0282-YC | Mouse Anti-NOTCH3 Recombinant Antibody (clone 122M23) | ELISA, IHC, WB | Mouse IgG |
| HPAB-M0577-YC | Human Anti-NOTCH3 Recombinant Antibody (HPAB-M0577-YC) | FC, WB, Inhib, FuncS | Human IgG1, κ |
| HPAB-M0578-YC | Human Anti-NOTCH3 Recombinant Antibody (HPAB-M0578-YC) | FC, WB, Inhib | Human IgG |
| HPAB-0754-WJ | Human Anti-NOTCH3 Recombinant Antibody (clone N37) | ELISA | Human IgG |
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