Mouse Anti-NR1H2 Recombinant Antibody (clone 5C11D3) (CAT#: VS7-0425-WR500)
This product is a mouse IgG2b antibody that binds to human NR1H2. The antibody 5C11D3 recognizes specific amino acid domain of human NR1H2 (AA:1-200). It can be used for ICC and FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5446/1871_A8_18_cr5b719ffa04575_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5446/1871_A8_18_cr5b719ffa04575_selected.jpg
Figure 2 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70212/1476_G5_2_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70212/1476_G5_2_selected.jpg
Figure 3 Cerebellum
(Purkinje cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56838/133028_B_8_8.jpg
(Purkinje cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56838/133028_B_8_8.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56838/133028_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56838/133028_A_6_6.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131408-NR1H2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131408-NR1H2
Specifications
- Immunogen
- Recombinant human NR1H2 (AA:1-200) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human NR1H2
- Species Reactivity
- Human
- Clone
- 5C11D3
- Molecule Class
- Nuclear Receptor
- Applications
- ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NR1H2; LX Receptor Beta; Liver X Receptor Beta; Ubiquitously-Expressed Nuclear Receptor; Nuclear Receptor NER; Steroid Hormone-Nuclear Receptor NER; LXRB; RIP15; Nuclear Orphan Receptor LXR-Beta; LXR-B; Liver X Nuclear Receptor Beta; Liver X Receptor-Beta
- Gene ID
- 7376
- UniProt ID
- P55055
- Long Name
- Nuclear Receptor Subfamily 1, Group H, Member 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Isopeptide bond; Ubl conjugation
- Protein Refseq
- NP_001243576.2
- Function
- Activator; DNA-binding; Receptor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2679z | Mouse Anti-NR1H2 Recombinant Antibody (clone 3B5) | ELISA, WB | Mouse IgG1 |
| MOB-1626MZ | Recombinant Mouse Anti-Human NR1H2 Antibody | WB | Mouse antibody |
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