Mouse Anti-NRP1 Recombinant Antibody (clone 5B1E11)
CAT#: VS7-0425-WR469
This product is a mouse IgG1 antibody that binds to human NRP1. The antibody 5B1E11 recognizes specific amino acid domain of human NFE2L2 (AA: 356-589). It can be used for WB and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate granular positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/64887_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/64887_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/64887_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/64887_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/64887_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30278/64887_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (fHDF/TERT166, hTERT-RPE1, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099250-NRP1
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Specifications
- Immunogen
- Recombinant human Neuropilin-1 (AA: 45-59) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NRP1
- Species Reactivity
- Human
- Clone
- 5B1E11
- Molecule Class
- Neuropilins
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Vesencumab;1205533-60-3;Genentech Anti-NRP1;RG7347;MNRP1685A;NRP1;neuropilin 1;neuropilin-1;CD304;NRP;VEGF165R;transmembrane receptor;vascular endothelial cell growth factor 165 receptor;NP1;BDCA4;DKFZp781F1414;DKFZp686A03134;
- Gene ID
- 4780
- UniProt ID
- O14786
- Long Name
- Neuropilin 1
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane; Mitochondrion; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Heparan sulfate; Phosphoprotein; Proteoglycan
- Protein Refseq
- NP_001019799.2
- Function
- Developmental protein; Heparin-binding; Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
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- Product Name
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