Mouse Anti-NT5E Monoclonal Antibody (VS7-0425-WR510)
CAT#: VS7-0425-WR510
This product is a mouse IgG antibody that binds to human NT5E. The antibody can be used for IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
Normal Tissue
Figure 7 Cervix
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
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Specifications
- Immunogen
- Recombinant human NT5E fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NT5E
- Species Reactivity
- Human
- Molecule Class
- Plasma Membrane Protein
- Applications
- IHC-P, IF, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Calcification of joints and arteries (CALJA)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NT5E; 5-Nucleotidase, Ecto (CD73); 5' -Nucleotidase, Ecto (CD73); NTE; 5 Nucleotidase (CD73); NT5; CD73; NT; CD73 Antigen; CALJA; E5NT; Purine 5-Prime-Nucleotidase; Ecto-5-Nucleotidase; 5-NT; EC 3.1.3.5; 5-Nucleotidase
- Gene ID
- 4907
- UniProt ID
- P21589
- Long Name
- 5'-Nucleotidase Ecto
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein
- Protein Refseq
- NP_001191742.1
- Function
- Hydrolase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NT5E"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1936z-F(E) | Recombinant Anti-Human NT5E Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| HPAB-N0374-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0374-YC-F(E)) | ELISA, FC, Inhib | Mouse Fab |
| HPAB-N0375-YC-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0375-YC-F(E)) | ELISA, FC, Inhib | Human Fab |
| HPAB-N0196-YC-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0196-YC-F(E)) | FC | Human Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1936z-S(P) | Recombinant Anti-Human NT5E Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| HPAB-N0374-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0374-YC-S(P)) | ELISA, FC, Inhib | Mouse scFv |
| HPAB-M0172-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0172-YC-S(P)) | FC, Inhib | Mouse scFv |
| HPAB-M0173-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0173-YC-S(P)) | FC, FuncS | Mouse scFv |
| HPAB-N0196-YC-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0196-YC-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-593MZ-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (TAB-593MZ-F(E)) | FC | Human Fab |
| TAB-594MZ-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (TAB-594MZ-F(E)) | FC | Human Fab |
| TAB-595MZ-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (TAB-595MZ-F(E)) | FC | Human Fab |
| TAB-596MZ-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (TAB-596MZ-F(E)) | FC | Human Fab |
| TAB-597MZ-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (TAB-597MZ-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1799CQ | Recombinant Mouse Anti-NT5E Antibody (NEUT-1799CQ) | FC, IHC-Fr, IF, IP, Inhib | IgG2a, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2503 | Hi-Affi™ Recombinant Rabbit Anti-NT5E Monoclonal Antibody (DS2503AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0374-YC | Mouse Anti-NT5E Recombinant Antibody (HPAB-N0374-YC) | ELISA, FC, Inhib | Mouse IgG |
| HPAB-N0375-YC | Human Anti-NT5E Recombinant Antibody (HPAB-N0375-YC) | ELISA, FC, Inhib | Human IgG |
| MRO-0315-CN | Recombinant Rabbit Anti-NT5E Monoclonal Antibody (JM11-40) | WB, IHC | Rabbit IgG |
| FN-029CQ | Mouse Anti-NT5E Recombinant Antibody (clone AD2) | FC, Block | Mouse IgG1 |
| FAMAB-1684CQ | Rabbit Anti-Nt5e Recombinant Antibody (clone YDBYT 208.3) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-441CQ | Afuco™ Anti-NT5E ADCC Recombinant Antibody, ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1442 | AbPlus™ Anti-Nt5e Magnetic Beads (VS-0724-YC1442) | IP, Protein Purification | |
| VS-0724-YC1493 | AbPlus™ Anti-NT5E Magnetic Beads (VS-0724-YC1493) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC844 | CytoStream™ Mouse Anti-NT5E Recombinant Antibody (VS13-YC844) | WB, IHC-P, ICC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY59 | Human Anti-NT5E (clone Uliledlimab) scFv-Fc Chimera | FC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC110 | Recombinant Anti-NT5E Vesicular Antibody, EV Displayed (VS-0425-YC110) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5002 | Anti-NT5E Immunohistochemistry Kit | IHC | |
| VS-0525-XY5003 | Anti-Mouse NT5E Immunohistochemistry Kit | IHC | |
| VS-0525-XY5004 | Anti-Rat NT5E Immunohistochemistry Kit | IHC |
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