Mouse Anti-NTRK1 Monoclonal Antibody (VS7-0425-WR511)
CAT#: VS7-0425-WR511
This product is a mouse IgG antibody that binds to human NTRK1. The antibody recognizes specific amino acid domain of human Trk A (aa33-423). It can be used for WB, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76570/1680_E8_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4606/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198400-NTRK1
❮
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Specifications
- Immunogen
- Recombinant human Trk A (aa33-423) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NTRK1
- Species Reactivity
- Human
- Molecule Class
- Neurotrophic Tyrosine Kinase Receptor (NTKR) Family
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Congenital insensitivity to pain with anhidrosis (CIPA)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NTRK1; neurotrophic tyrosine kinase, receptor, type 1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; high affinity nerve growth factor receptor; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase
- Gene ID
- 4914
- UniProt ID
- P04629
- Long Name
- Neurotrophic Receptor Tyrosine Kinase 1
- Cellular Localization
- Cell membrane; Endosome; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001007793.1
- Function
- Developmental protein; Kinase; Receptor; Transferase; Tyrosine-protein kinase
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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