CytoStream™ Rabbit Anti-NTRK1 Recombinant Antibody (VS13-YC846)

Name: CytoStream™ Rabbit Anti-NTRK1 Recombinant Antibody (VS13-YC846)
SKU: VS13-YC846
Rating: 5 (1 reviews)

CAT#: VS13-YC846

The CytoStream™ Rabbit Anti-NTRK1 Recombinant Antibody is specific for human, mouse and rat NTRK1. NTRK1 encodes a kinase receptor in neuron differentiation, with mutations causing insensitivity to pain and cancer linkage, possessing characterized splice variants. The anti-NTRK1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line SH-SY5Y

Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76570/1680_E8_5_selected.jpg

Subcellular Location Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/ihc_selected.jpg

Subcellular Location Figure 3 IHC staining of human cerebellum

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4606/ihc_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Glial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_B_9_5.jpg

Normal Tissue Figure 5 Colon

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_A_9_3.jpg

Normal Tissue Figure 6 Testis

Elongated or late spermatids
Staining:

Medium

Intensity: Moderate
Quantity:>75%

Pachytene spermatocytes
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%

Round or early spermatids
Staining:

Medium

Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35799/129189_A_6_6.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enriched (HMC-1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198400-NTRK1

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Specifications

Immunogen
Recombinant human TrkA, aa 747-796.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat NTRK1

Species Reactivity
Human, Mouse, Rat

Applications
WB, ICC, IF, IHC-P, IP, FC

Conjugate
NONE

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

Preservative
0.05% Sodium azide

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Shipping with ice pack.

Applications

Application Notes
Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100

Target

Alternative Names
Neurotrophic Receptor Tyrosine Kinase 1; Neurotrophic Tyrosine Kinase, Receptor, Type 1; High Affinity Nerve Growth Factor Receptor; TRK1-Transforming Tyrosine Kinase Protein; Tropomyosin-Related Kinase A; Tyrosine Kinase Receptor A; EC 2.7.10.1; P140-TrkA; Gp140trk; Trk-A;

Gene ID
4914

UniProt ID
P04629

Long Name
Neurotrophic Receptor Tyrosine Kinase 1

Cellular Localization
Cell membrane; Early endosome membrane; Late endosome membrane

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS13-YC846.pdf

MSDS

MSDS - VS13-YC846.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
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