Mouse Anti-Nucleosome Recombinant Antibody (clone 2E1) (CAT#: FAMAB-0232YC)

Privided is a monoclonal antibodies binding to nucleosomes, total histones and to the H2A-H2B dimers but not to
individual histones or DNA. The clone 2E1 was selected based on their capacities to react with nuclei in an indirect immunofluorescence assay using cytospin preparations of HEp-2 cells as the substrate.


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ELISA

Figure 1 Inhibition of mAb binding.

Figure 1 Inhibition of mAb binding.

Microtiter plates were coated with H2A-H2B dimer and incubated with mAb 3F6 (▽), 1A6 (●), 3B10 (○), 2E1 (▼) and various concentrations of H2AH2B dimers.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a, κ
  • Specificity
  • Human Nucleosome
  • Species Reactivity
  • Human
  • Clone
  • 2E1
  • Applications
  • ELISA, IF
  • Related Disease
  • SLE

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Histone-DNA complex

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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